Enzyme concentrations are often characterized by 'units' of activity per volume. One enzyme we will be...

Enzyme concentrations are often characterized by 'units' of activity per volume.

One enzyme we will be using, Sbfl, is supplied in a concentration of 10,000 units/mL

a. How many are there in 1 uL?

b. How much volume would you need for 20 units?

c. We have 500 units total, how much volume is that?

Solutions

Expert Solution

(a) Given concentration is 10,000 units/mL

That means 1 mL contains 10,000 units

1 uL = 10-6 mL contains (10,000 units*10-6 mL) / 1 mL = 10-2 units

(b) Given concentration is 10,000 units/mL

That means 10,000 units requires 1 mL

20 units require (20 units*1 mL) / 10,000 units = 0.002 mL

That means 10,000 units requires 1 mL

500 units require (500 units*1 mL) / 10,000 units = 0.05 mL

queen_honey_blossom answered 2 years ago

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