multiple choice questions .( questions may have more than answer ) 1- Rough endoplamatic reticulum :...

multiple choice questions .( questions may have more than answer )

1- Rough endoplamatic reticulum :
a. does not contain ribosoms .
b. contains a small amount of RNA
c. is responsible for lipid synthesis .
d. is responsible for protein synthesis .

2. karyotype :
a. can be performed only on the basis of dividing cell
b. requires sterile condition .
c. can be performed on the basis of paraffin embedded tissue
d. enables detection of structural chromosomal alteration .

3. Southern blotting :
a. is performed on the basis of genomic DNA
b.is performed on the basis of PCR product
c. is method used in large mutation detection
d. is a method used in detection of proteins

4. tumor suppressors genes :
a. are mutated form of proto-oncogenes
b.one mutated allel is enough to cause cance
c. encode proteins that repair our DNA .
d. Rb and P53 are examples of such genes .

5 . which of the following can lead to loss of gene function :
a. A missense mutation
b. A silent mutation
c. A change from T to C in TATA box
d. A nonsense mutation .

Expert Solution

1) The correct option is d. is responsible for protein synthesis.

Ribosomes are attached to the membrane of the ER, therefore it is known as rough endoplasmic reticulum. It is very important in the synthesis and packaging of proteins.

2) The correct option is d. enables detection of structural chromosomal alteration .

Karyotype is the number and form of chromosomes in the nucleus of a eukaryotic cell. It is the complete set of chromosomes in a species or in an individual organism. Chromosomes show a banded pattern when treated with some stains. Bands are alternating light and dark stripes, which show along the lengths of chromosomes. Unique banding patterns are used to identify chromosomes and diagnose chromosomal aberrations, which include chromosome breakage, loss, duplication, translocation or inverted segments.

3) The correct option is a. is performed on the basis of genomic DNA .

Southern blot is a process, which is used in detection of a specific DNA sequence in DNA samples. This blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

4) The correct option is d. Rb and P53 are examples of such genes .

The first tumor-suppressor protein is Retinoblastoma protein (pRb) in human retinoblastoma and another important tumor suppressor is the p53 tumor-suppressor protein encoded by the TP53 gene.

gladiator answered 2 years ago

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