Question

In: Biology

Describe how molecules of DNA can have different electrophoretic mobility.

Describe how molecules of DNA can have different electrophoretic mobility.

Solutions

Expert Solution

The combined results that indicate that DNA electrophoretic mobilities are determined by the interplay of three factors: the relative size of the DNA molecules with respect to the effective pore size of the matrix, the effect of the electric field on the matrix, and specific interactions of the matrix with the DNA molecules.

DNA electrophoretic mobilities are highly dependent on the nature of the matrix that in which the separation take place.

This review describes the effect of the matrix on DNA separations in agarose gel, polyacrylamide gels and solutions containing entangled linear polymers, correlating the electrophoretic mobilities with information obtained from other types of studies. DNA mobilities in various sieving media are determined by the interplay of three factors: the relative size of the DNA molecule with respect to the effective pore size of the matrix, the effect of the electric field on the matrix, and specific interactions of DNA with the matrix during electrophoresis.


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