Question

In: Chemistry

1- why is method development easier with gradient elution chromatography than isocratic separations?2- in some detail...

1- why is method development easier with gradient elution chromatography than isocratic separations?2- in some detail explain why one would use elevated temperatures in liquid chromatography ? 3- what is DryLab and why use it? please give me refrences?

Solutions

Expert Solution

  1. Isocratic elution is where the composition of the mobile phase is unchanged during the entire elution process. Gradient elution is where the solvent strength of the mobile phase in increased during the elution starting with a solvent of relatively low solvent strength. Method development is easier with gradient elution chromatography than isocratic elution chromatography. Because, gradient elution decreases the retention of the later-eluting components so that they elute faster, giving narrower (and taller) peaks for most components.
  2. In recent years, the use of elevated temperatures in liquid chromatography has become more familiar, although many people still fear to increase the temperature because of unwanted side effects. In most laboratories, separations are usually performed at room temperature. In this setting, elevated temperature liquid chromatography would mean the temperature ranges from 40oC-60oC, whereas high temperature liquid chromatography extends from 60oC to 200oC.
  3. Dry lab is an educational exercise in which most of the hand on components a particular lab procedure are performed with the exception of those requiring use of a sink and biohazardous disposal.

Related Solutions

Gradient conditions and methods development a) why is method development easier with gradient elution chromatography than...
Gradient conditions and methods development a) why is method development easier with gradient elution chromatography than isocratic separations? b) in some detail explain why one would use elevated temperatures in liquid chromatography? c) what is DryLab and why use it? Please provide reference
Discuss 1) The Sanger method and 2) Affinity chromatography
Discuss 1) The Sanger method and 2) Affinity chromatography
         Gas chromatography questions 1. why is GC separation more efficient than a fractional distillation? 2....
         Gas chromatography questions 1. why is GC separation more efficient than a fractional distillation? 2. what characteristics must the liquid stationary phase have? 3. how do the flow rate of the carrier gad and the column temperature affect the retention time of a compound on a GC column? 4. Describe a method for identifying a compound using GC analysis
1. Why is the steady state concentration gradient linear in equimolecular counter diffusion? 2. Explain why...
1. Why is the steady state concentration gradient linear in equimolecular counter diffusion? 2. Explain why the dissolution of a crystalline particle in a solution can be speeded up by (a) stirring the solution and (b) by crushing the particle, from a mass transfer perspective.
1) List the three steps of test driven development and explain, in detail, why these three...
1) List the three steps of test driven development and explain, in detail, why these three steps are used. 2) Explain why the order of the steps is so important. 3) For each of the following data types, explain what boundaries might be relevant to testing. Also, explain what values you would consider testing for parameters of this type. Justfiy your answers. boolean int String List Optional Point Map
Pick an industry other than the financial sector that requires regulation. 1. describe in some detail...
Pick an industry other than the financial sector that requires regulation. 1. describe in some detail and identify the market 2. model it to make sure to set it within the IE terminology 3. clarify why it requires regulation 4. what sort of regulation should be applied to this industry and why? 5. what difficulties might face in applying regulation to this industry. P.S: this is a government and business class question.
1. Why are some countries richer than others according to Adan Smith (1776) 2) Loanable Funds...
1. Why are some countries richer than others according to Adan Smith (1776) 2) Loanable Funds Market 2.1 Graph Supply Curve   Explain in detail. 2.2) Graph Demand Curve Explain in detail 2.3) Graph Supply and Demand in interaction Explain Price equilibrium - Interest rate 2.4) Graph Supply and Demand when there is a Saving Incentive. 2.5) Graph Supply and Demand when there is a Tax Incentive.
1. In some detail, explain why we (as humans) should not rely on the natural selection...
1. In some detail, explain why we (as humans) should not rely on the natural selection for the ∆32 allele to produce universal or near-universal resistance to the HIV virus 2. From an evolutionary perspective, what is meant by “fitness”? 3. List three different types or categories of mutations, describe briefly how each occurs, and the consequences of each regarding genetic variations 4. Although non-random mating does not itself change allele frequencies so technically is not a mechanism of evolution,...
1. why might some antibiotics show greater evidence of bacterial resistance than others? 2. do any...
1. why might some antibiotics show greater evidence of bacterial resistance than others? 2. do any of the results obtained suggest that bacterial genes that confer resistance to certain antibiotics also work against chemically similar antibiotics? 3. Staphylococcus epidermidis is gernally not considered a harmful species of bacteria; in fact, it may be beneficial to humans to have this organism living on our skin. even so, why should we be concerned that this species shows resistance to a variety of...
1. Perform two iterations of the gradient search method on f(x,y)= x^2+4xy+2y^2+2x+2y. Use (0,0) as a...
1. Perform two iterations of the gradient search method on f(x,y)= x^2+4xy+2y^2+2x+2y. Use (0,0) as a starting point. Please find the optimal λ* by taking the derivative and setting it equal to 0.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT