In: Physics
A rod-shaped DNA molecule (rod) of length 250 nm undergoes a
conformational change to a closed-circular (cc) form.
(a) Assuming that scattering light has a wavelength of 488 nm,
calculate the ratio of scattering intensities of each of these
conformations (i.e. Irod/Icc) when q = 20°, 45°, and 90°. Use the
following values: M = 4 x 106 g/mol ns = 0.556 cm3/g Rg = 117.0
nm
(b) Based upon this answer, what is the best value of q to use to
study the conformational changes of DNA? (20 points)
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