In: Biology
ANSWER )
PLEASE GO THROUGH RED BOLD HIGHLIGHTED ANSWER. THE BLACK BOLD IS JUST AN EXPLANATION
M1 X V1 = M2 X V2
M1 = 3 M (STOCK SOLUTION CONCENTRATION)
M2 = 0.03M ( REQUIRED SOLUTION CONCENTRATION)
V2 = 100 ( REQUIRED VOLUME )
V1 = (STOCK VOLUME)
APPLYING THIS FORMULA 3 X V1 = 0.03 X 100
= V1 = 0.03 X 100/ 3 = 1 ML
SINCE WE DON'T HAVE CYLINDER TO MEASURE 1 ML
SO WE WILL INCREASE THE REQUIRED VOLUME ( V2 ) TO 1000 ML ACCORDINGLY WE WILL CALCULATE AGAIN
M1 X V1 = M2 X V2
3 X V1 = 0.03 X 1000
V1 = 10ML
10ML CAN BE MEASURED FROM MEASURING CYLINDER
SO NOW FROM 3 M AQUEOUS GLUCOSE STOCK SOLUTION WE WILL TAKE 10 ML AND ADD UP IN 990 ML OF WATER ( 100 ML WATER X 9 TIMES AND 90 ML WATER X 1 TIME )
THIS WILL MAKE 1000 ML OF 0.03 M GLUCOSE SOLUTION
NOW MEASURE 100 ML OF SOLUTION OF 0.03M GLUCOSE SOLUTION AS YOU REQUIRED ACCORDING TO QUESTION
NOTE WE COULD HAVE INCREASE OUR V2 VOLUME TO 500 BUT IT MAY LEAD TO DISCREPANCY AND NOT ACCURATELY MEASURE SO WE HAVE TAKEN 1000ML