In: Chemistry
Hello. I am attempting to test kidney stone solubility by using titration methods. I want to set up an experiment that incorporates lemon juice and EDTA solution to dissolve an artificial kidney stone. I need to find a procedure that includes: lemon juice, kidney stone(calcium oxalate), EDTA, and titration methods. Please be as detailed as possible with step-by-step instructions. Thank you.
Investigation of Kidney Stones
To test how long it would take for the calcium phosphate to dissolve in lemon juice.
The most common types of kidney stones are:
Calcium Oxalate: Accounts for 70% of all kidney
stones (CaC2O4)
We will synthesize first artificial kidney stones.The procedure is
Calcium Oxalate:
o Dissolved 4.33g of Calcium Chloride (CaCl2) in 10 mL of
water
o Dissolved 5.23g of Sodium Oxalate (Na2C2O4) in 10 mL of
water
o We then mixed the two aqueous solutions to form a
precipitate
CaCl2 (aq) + Na2C2O4 (aq) --> CaC2O4 (s) + 2NaCl (aq)
Net ionic: Ca2+ (aq) + C2O42- (aq) --> CaC2O4 (s)
White precipitate formed, solution was cloudy
Solid calcium oxalate was formed
After Vacuum filtration, centrifuging, and dilution:
2.76g solid calcium oxalate was obtained
Percent yield: 220%
The next step is to dissolve kidney stone.We will use Lemon juice
0.131g CaC2O4 (s) dissolved in 50 mL lemon juice in 30 min.
Dissolving stones