EMB 1. What makes EMB selective? 2. What does EMB select for? 3. What makes EMB...

EMB
1. What makes EMB selective?
2. What does EMB select for?
3. What makes EMB differential?
4. What does EMB differentiate between?
5. What pH indicator is used in EMB?

6. What causes the indicator to change color?

Expert Solution

Ans-1.) Eosin dye and methylene blue makes the medium selective as they inhibit the growth of gram-positive bacteria to a limited degree .

Ans-2.) It inhibits the growth of gram positive bacteria ,therefore selects for gram negative bacteria .

Ans-3.) The dyes also serve as differential indicators for the products of fermentation. Lactose and sucrose provide a carbohydrate source. The production of acid from lactose- or sucrose-fermentation results in the eosin-methylene blue dye complex being taken up by bacterial cells to produce a brown to blue-black colony appearance.

Ans-4.)The Preparation provides a clear distinction between colonies of lactose and non-lactose-fermenting microorganisms.

Ans-5.) Eosin Y and methylene blue are pH indicator dyes.

Ans-6.) Vigorous fermenters of lactose or sucrose will produce sufficient quantities of acid , which combine with eosin-methylene blue complex to form a dark purple precipitate at low pH.

gladiator answered 2 years ago

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