How do you prepare 25 ml of 8.5 mM NaCl from a 20% NaCl stock? (Hint:...

How do you prepare 25 ml of 8.5 mM NaCl from a 20% NaCl stock? (Hint: convert 20% to g/ml then to molar)

Expert Solution

20% NaCl means , in 100 gm of solution there are 20 gm NaCl.
water =80 gm
since density of water in 1 gm/ml
volume of solution = 80 mL
molar mass of NaCl = 23+35.5 = 58.5 gm
number of moles of NaCl = mass / molar mass
= 20/58.5
=0.342
volume of solution = 80 mL = 0.08 L
Molarity = mol /volume = 0.342/0.08 = 4.275 M
From this solution we need to prepare 25 mL of 8.5 mM solution
use dilution formula:
Mi*Vi = Mf*Vf
4.275 * V = 8.5*10^-3 * 25
V = 0.05 mL
So, volume of original solution that needs to be taken is just 0.05 mL
Take 0.05 mL of original solution and add enough water to make it 25 mL and you will get your final required solution

queen_honey_blossom answered 2 hours ago

Write how to prepare the following: 1. 20.0 mL of 25 mM lysine from solid 2....

Write how to prepare the following: 1. 20.0 mL of 25 mM lysine from solid 2. 1 L of 0.5 M of NaCl from a 6 M stock of HCl 3. 10 mL of 0.60 M HCl from a 6 M Stock HCl

Describe how you would prepare 2 mL of solution containing NaCl, MES buffer, and water. NaCl...

Describe how you would prepare 2 mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100 M and the working concentrations required is 20 mM. To make 2 mL of solution calcualte the amount of water you would add. Please Show Work

How many grams of NaCl are needed to prepare 30 mL of a 0.87 M NaCl...

How many grams of NaCl are needed to prepare 30 mL of a 0.87 M NaCl solution?

A solution is prepared that contains 10 mM NaCl, 20 mM CaCl2, 20 mM BaCl2, and...

A solution is prepared that contains 10 mM NaCl, 20 mM CaCl2, 20 mM BaCl2, and 50 mM NiNO3. Of course, all these salts completely dissolve to their respective cations and anions. The pH is 7.0. (a) Calculate the ionic strength of the solution. (b) Calculate the activity coefficients for H+, Cl-, and Ba2+ (use the Guntenberg approximation because the solutions do, though note there are a few other equations and a graph that can be used at your discretion...

1. How many grams of NaCl are required to prepare 500. ml of 1.20M NaCl solution?...

1. How many grams of NaCl are required to prepare 500. ml of 1.20M NaCl solution? 2. ) Write the balanced equation for the reaction that occurs when sulfuric acid, H2SO4 is titrated with the base, sodium hydroxide, NaOH. Sodium sulfate, Na2SO4 is the salt produced. 3. SO2 gas is in a 2.0L flask at STP (0 o C, 1 atm). A. Using the Ideal Gas Law, determine the concentration of the gas in moles/L B. Calculate the density of...

100 mL of 0.5M NaCl in 25 mM sodium phosphate, pH 8.0. Provide calcualtions and explain...

100 mL of 0.5M NaCl in 25 mM sodium phosphate, pH 8.0. Provide calcualtions and explain how the 0.5M NaCl in 25 mM sodium phosphate buffer, pH 8, will be prepared.

Show calculations AND explain how to prepare 275 mL of 1.0 M NaCl from solid sodium...

Show calculations AND explain how to prepare 275 mL of 1.0 M NaCl from solid sodium chloride. and Show calculations AND explain how to prepare 275 mL of 1.0 M NaCl from a 5.0 M stock solution of sodium chloride

(a) How do you prepare a 0.094% NaCl solution? (b) Tell whether the solution in the...

(a) How do you prepare a 0.094% NaCl solution? (b) Tell whether the solution in the medium outside of the cell is hypertonic, isotonic or hypotonic to the cell. # Inside the Cell Outside of the Cell in the Medium 1 0.009% NaCl 0.009% NaCl 2 0.076% NaCl 0.077& NaCl 3 0.02% NaCl 0.002% NaCl 4 0.09% NaCl 0.009% NaCl

Describe how would you prepare the GTE (50 mM Glucose, 20 mM Tris, EDTA) Resuspension Solution...

Describe how would you prepare the GTE (50 mM Glucose, 20 mM Tris, EDTA) Resuspension Solution for use in a mini-prep. You have the following stock solutions: 0.5 M glucose, 0.2 M Tris-HCl (pH 8.0), 0.5 M EDTA (pH 8.0). How would you prepare 150 mL of GTE re-suspension solution using the above stocks? Also, describe how to prepare the solution in appropriate container and aseptic technique.

Using the stock solution provided (7% NaCl) and water, prepare the following solutions: i) 1 mL...

Using the stock solution provided (7% NaCl) and water, prepare the following solutions: i) 1 mL of NaCl solution with an osmolarity of 50 mOsM ii) 1 mL of NaCl solution with an osmolarity of 300 mOsM iii) 1 mL of NaCl solution with an osmolarity of 1200 mOsM

Question