This protein sequence forms an antiparallel coiled-coil. Place the peptides onto a helical wheel diagram and...

This protein sequence forms an antiparallel coiled-coil. Place the peptides onto a helical wheel diagram and show which faces interact in the coiled-coil. Identify key interactions that stabilize the coiled-coil.

I L R L E R E I E D L Q K M K

Expert Solution

Ans: Isoleucine (non polar), leucine (non polar), Arginine (+vely charged), leucine, Glutamate (-vely charged) , Arginine, Glutamate, Isoleucine, Glutamate, Aspertate (-vely charged), leucine, Glutamine (polar), Lysine (+vely charged), Methionine (non polar), Lysine

I L R L E R E I E D L Q K M K

From the sequence it is identified as leucine zipper. In which every seventh aminoacid is leucine, they interact hydrophobically and stabilise the coiled-coil. In the ring inner residues are coil 1 and outer residues are coil 2. I will start describing the sequence from left to right (from the second loop). KMKQLD forms coil 2. ILRLEREIE forms coil 1 these two coils interact by 4^th leucine on coil 1 (from left to right on the pepetide) and 11^th leucine on the 2^nd coil. Other than this DI (polar outerside of coil vs nonpolar innerside), RQ (uncharged slightly nonpolar innerside vs polar outerside of coil), EM (polar vs nonpolar), KR (both slightly polar), KL (slightly polar outerside of coil vs nonpolar innerside) interactions help the coiled coil stability

gladiator answered 2 years ago

. In the primary sequence of a protein, a valine is substituted in place of a...

. In the primary sequence of a protein, a valine is substituted in place of a glutamic acid. Which of the following statements is true? a. This is a transition from a non-polar amino acid to a polar amino acid. b. This will affect the secondary protein structure. c. This is a transition from a hydrophobic amino acid to a hydrophilic amino acid. d. This will have no effect on the protein. e. This is a silent mutation.

In the protein adenylate kinase, the C-terminal region is a helical, with the sequence Val-Asp-Asp-Val-Phe-Ser-Gln-Val-Cys-Thr-His-Leu-Asp-Thr-Leu-Lys. a)...

In the protein adenylate kinase, the C-terminal region is a helical, with the sequence Val-Asp-Asp-Val-Phe-Ser-Gln-Val-Cys-Thr-His-Leu-Asp-Thr-Leu-Lys. a) Underline the hydrophobic amino acids in the sequence. b) If you identified the correct amino acids you will notice that there is a periodicity of hydrophobic amino acids. What is this periodicity and why would this occur?

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This protein sequence forms an antiparallel coiled-coil. Place the peptides onto a helical wheel diagram and...

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. In the primary sequence of a protein, a valine is substituted in place of a...

In the protein adenylate kinase, the C-terminal region is a helical, with the sequence Val-Asp-Asp-Val-Phe-Ser-Gln-Val-Cys-Thr-His-Leu-Asp-Thr-Leu-Lys. a)...