Question

Biochemistry: "I provide radiolabeled malonyl-CoA to liver cells."

Base on the sentence above, create a question (relatives to metabolic pathway in cell: gluconeogenesis, PHD, TCA cycle). After that, answer to your own question that you just made

P/s: it's a complete question already. The requirement is: You have to think and create/make up your own question that relates to the sentences, then answer to the question you just made up.

Answer 1

Q:-- Detect the fate of Malonyl CoA which is radiolabeled in a cell. How can we use such radiolabeled malonyl CoA to understand biochemically pathway.Comment on it.

And:--It is coenzyme A derivative of Malonyl acid.

Function -- chain elongation in Fatty acid biosynthesis and polyketide synthesis.

Malonic acid is Krebs cycle inhibitor and barrier in ATP production.

Fate of Radiolabeled Malonyl CoA : Fate of this molecule can be understood by radiolabeled C14 isotope techniques and it can be used to Detect biosynthesis of Fatty acid and polyketide synthesis as well as Pathopysiology of such molecule.

1). In Biosynthesis:--Radiolabeled Malonyl CoA is used in a cell and this molecule gives off two carbon unit to Fatty acid to be elongate and among these C---C*. One is Radiolabeled with C14 type in place of normal C. Here in such a way This radiiradiolab molecule(malonyl CoA) is used to detect pathway of polyketide biosynthesis.

2). In Pathopysiology of Malonyl CoA :-- Without the activity of Melonyl CoA decarboxylase, cellular CoA increases so dramtically at the end it is instead broken down by an unspecific short chain acetyl CoA hydrolase which produces malonic acid and CoA. Malonic acid(with C* isotope from Melonyl CoA) inhibit Krebs cycle in such condition, the cells to make ATP, are forced to increase glycolysis and lactic acid is produced by product. So increase in lactic acid and malonic acid lowers the PH of blood and causes lactic and malonic aciduria(acidic urine). These are very rare cases, as fewer than 20 cases have been reported. These malonyl CoA bad effect traced by Incorporated C14(C*) in malonyloA and then Malonic acid.

So these were experiments by which fate of melonyl CoA in metabolism as well as in disease observed.