Question

When analyzing Raman spectra, we use an orgin program to substract the baseline in order to eliminate the fluorescence background of each material. I wonder what the baseline is based on.

Answer 1

Samples may exhibit Raman spectra with varying degrees of fluorescence or thermal background. Providing that there is sufficient Raman signal ‘on top’ of the sloping background, the baseline may be subtracted to yield a spectrum with a ‘flat’ baseline. In some cases, the measurement can be re-performed with an alternative excitation wavelength to more effectively remove the effects of fluorescence.

The following methods are available :

Intelligent fitting : default intelligent automated Option.

Through fixed points: User controls point position (XY) and baseline types.

Through chosen points on each spectrum : user controls X point which the baseline travels through for each spectrum within the
dataset.

Through Whole spectrum : Automatic fitting with no in-built intelligence.

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*(You can read more about the process of subtraction, you may refer the any manual about the Raman Spectroscopy.)

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