Which of the following is(are) not cis-acting? a. lacI b. lacO c. TATA box d. Enhancers e. Items a and b are not cis-acting
Which of the following is(are) not cis-acting? a. lacI b.
lacO c. TATA box d. Enhancers e. Items a and b are not cis-acting
f. There is not enough information to determine this
Solutions
Expert Solution
4. The correct option is
............................a.lacI
The components of transcription are termed as cis acting and
transacting.
Cis acting is the structural portion of a gene which is
required for gene expression.
Trans acting factors are the proteins which associate with Cis
acting factors to control gene expression.
Cis acting factors can be located in a promoter or an
enhancer.
Cis acting elements are located in non coding regions of
DNA.
A single cis element is sufficient to produce a regulatory
response.
TATA box is a promoter sequence, and it
indicates where the genetic sequence could be read and decoded in a
DNA.
Transcription factors associate with the TATA box to promote
the transcription of a gene.
Enhancer is a region of DNA that bound
to transcription activators (mostly proteins) to activate
the transcription of a gene.
The operator present in the lac operon also forms as an example
of the Cis acting regulatory sequence.
Lac I is a repressor protein involved in the lactose
metabolism, LacI inhibits the expression of genes to transcribe the
protein coding.
Consider the cross: A/a; b/b; C/c; D/d; E/e x A/a; B/b; c/c;
D/d; e/e
a) what proportion of the progeny will phenotypically resemble
the first parent?
b) what proportion of the progeny will genotypically resemble
neither parent?
For each of the following, indicate if it is
cis-acting or trans-actingA regulatory gene that codes for a transcription factor
_______________A promoter _______________An operator _________________An enhancer _________________
Given the following knowledge base:
a <- b^c.
b <- d^e.
b <- g^e.
c <- e.
d.
e.
ƒ <- a^g.
Which of the following would be the trace of resolved atoms
assuming a bottoms-up proof procedure?
Select one:
a. {a,b,c,e,g}
b. {a,b,c,e,d}
c. {g,e,b,e,c,a}
d. None of these options
Constraint Satisfaction Problem (CSP) is consists of a set of
_________________.
Select one:
a. Variables, heuristics, and solutions
b. Variables, domains, and backtracking
c. Variables, domains, and constraints
d....
Eukaryotic gene regulation: transcription
initiation
Initiation & the transcription initiation complex (including
enhancers).
Promoter; TATA box; Conserved & variable regions in
promoters.
Eukaryotic enhancers. Why is it important that there are
multiple control elements in a single enhancer? What binds to the
control elements? How is it possible for a small number of
activator and transcription factor proteins to regulate a large
number of genes?
Transcription: Elongation & RNA polymerase. RNA polymerase
vs. DNA polymerase. Termination.
MyoD: What makes it...
Consider the lac operon in E. coli.
a. List the cis-acting elements and the trans-acting factors
involved in the regulation of its expression.
b. Indicate which Transacting factors interact with which
cis-elements.
c. Describe what the factors do when they are bound to their
respective cis–elements.
d. Indicate the inducer in this system and how it works.
Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting: A) β-galactosidase B) Operator site C) LacI repressor protein D) Lactose E) Permease F) Promoter site
For each of the following, indicate if it is cis-acting or
trans-acting
A regulatory gene that codes for a transcription factor
_______________
A promoter _______________
An operator _________________
An enhancer _________________
Describe the difference between cis-acting and trans-acting
elements. Also list whether the following elements are cis- or
trans-acting:
A) Beta-galactosidase
B) Operator site
C) LacI repressor protein
D) Lactose
E) Permease
F) Promoter site