Question

Proteasome is a protein complex that targets proteins that are missfolded and degrades them. Protein levels can also be regulated by degron directed degradation. The degrons are amino acid sequences that when accessible to degradation machinery in the cell targets proteins for degradation. T/F

ChiP-seq stands for Chromatin immunoprecipitation-sequencing. It is a technology that allows us to identify both cis-acting and trans-acting elements. T/F

In bacteria, gene mapping can be done by interrupting conjugation at one minute intervals or by measuring the cotransduction frequencies. T/F

Answer 1

Q1) Proteasome is a protein complex that targets proteins that are missfolded and degrades them. Protein levels can also be regulated by degron directed degradation. The degrons are amino acid sequences that when accessible to degradation machinery in the cell targets proteins for degradation. T/F
Ans: TRUE
Soln: Degron are portion of a protein that plays an important role in protein degradation. Degrons contain short amino acid sequences, structural motifs and exposed amino acids (commonly Lysine or Arginine) located anywhere in the protein.

Q2) ChiP-seq stands for Chromatin immunoprecipitation-sequencing. It is a technology that allows us to identify both cis-acting and trans-acting elements. T/F
Ans: TRUE
Soln: Chromatin immunoprecipitation combining with sequencing (ChIP-seq) and microarray (ChIP-chip) are employed in TF binding sites discovery under a given condition. For example, ABA-elicited transcriptional regulation for 21 ABA-related TFs were constructed by using ChIP-seq and RNA sequencing. ChIP-Seq delivers genome-wide profiling with massively parallel sequencing, generating millions of counts across multiple samples for cost-effective, precise, unbiased investigation of epigenetic patterns
Q3) In bacteria, gene mapping can be done by interrupting conjugation at one minute intervals or by measuring the cotransduction frequencies. T/F
Ans: True
Soln: In bacteria, gene mapping can be done by interrupting the conjugation within 1-3 minutes by conventional recombination methods. The number of genes dispatched from donor to recipient is proportional to the time interval for which conjugation is allowed in any conjugation experiment. By using this principle the technique called ‘interrupted conjugation technique’ is developed for mapping the bacterial genome.
By measuring the cotransduction frequency the percentage of cells that have inherited both genes can be identified. The higher their cotransduction frequency, the closer two genes must be to each other with this information the gene mapping can be done more precisely.