Question

During initiation of translation:

Question 6 options:

	both 70S and 30S ribosomal subunits must simultaneously recognize an mRNA to bind.
	the initiator tRNAmet binds to the A site of a ribosome.
	IF-3 must be recruited to the 30S ribosome in order for the 70S initiation complex to assemble.
	specific rRNA base pairs with a sequence in mRNA to position a ribosome at the start codon.
	there is no energy expenditure as the tRNA binding to mRNA is via complementary base pairing.

Answer 1

During translation

Initiation involves binding of small ribosomal subunit (30s) to mRNA and then is joined by 50s subunit. They don't bind simultaneously

The initiated tRNA binds directly to the P site

IF3 binds to 30S subunit and prevents premature reassociation of the large and small subunits of the ribosome.

Ribosomal binding site called is a small polypurine sequence upstream of initiation codon and is complementary to a region at the 3'end of the 16s rRNA

During translation GTP is hydrolysed when 50s subunit joins with 30s subunit to generate a complete ribosome