Describe the enzymes that are produced by the Operon

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Ans: An operon is a cluster of structural genes that are transcribed into a polycisstronic mRNA whose protein products are involve in a common biochemical pathway. the expression of the structural genes is regulated by a common mechanisams. A typical operon consists of the following components.

1 Structural genes-polycistronic mRNA

2 Promoter- Cis regulater; Non-transcribed

3 Operator- Cis regulater; Non-transcribed

4 Regulatory gene-Monocistronicwith it own promoter ; trans acting

Example: the lac operon was the first ever operon to be studied; the operon concept was proposed by Jacob & monod for the regulation of the prokaryotes gene expression.

Lac operon contain 3 strictural genes Lac Z, Lac Y and Lac A which produce three genes Beta galactosidase, Beta galactoside permease and transacetylase enzymes respectively.

Beta galactosidase: Lac Z gene encodes the enzyme Beta galactosidase. Beta galactosidase carries out a two distinct functions. It hydrolyses the Betagalactoside lactose into galactose and glucose.

Beta galactoside permease: Lac Y encodes the enzyme Beta galactoside permease. it is a ttansmembranal carrier protein. whose function is to transport the lactose from the medium into the bacterial cytoplasm.

Transacetylase : Lac A encodes the enzyme transacetylase, whose function is to carryout the acetylation by using an acetyl group donor.

gladiator answered 1 year ago

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