In: Biology
1.
Restriction enzymes (select all that apply):
Always produce sticky ends. |
Typically cleave at palindromic sequences 4-8bp long. |
Cleave invading viral DNAs. |
Are found in a wide variety of mammalian cell types. |
Are necessary for joining two DNA fragments together. 2. A polylinker is:
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1.ResticRestriction enzymes:-
Typically cleave at palindromic sequences 4-8bp long.
Cleave invading viral DNAs.
2.A polylinker is:-A synthetic fragment that contains multiple sites for restriction enzymes to cut the DNA.
3.plasmid vector typically has following features:-An origin of replication.
A selectable marker to ensure that the only cells containing the intact vector can grow.
Unique restriction sites for insertion of DNA.
4.DNA sequence of interest was digested and ligated into the middle of the AmpR gene region of pBR322 vector. The vector also contains a TetR gene which is not interrupted. After introducing the recombinat DNA into bacterial cells, results would be Growth on tetracyclin containing media.Because In the trnasfomed bacteria, tetracycline resistance gene is intact so it grows on tetracycline media.
5.following is true of genomic and cDNA libraries:-cDNA libraries contain sequences from expressed protein coding genes only.
Genomic libraries usually contain large fragments and are likely to be constructed in YAC or BAC vectors.
Genomic libraries contain fragments of genomic DNA that are generated by partial digests with restriction enzymes.
Last option is incorrect because cDNA library made from only mRNA. Not from all types of RNA.
7.Overexpression of proteins using bacterial vectors:-Requires a bacterial promoter to be present in the bacterial vector.