Question

In: Biology

Describe the colony morphologies you observed on the MacConkey (MAC), Nutrient and Horse Blood Agar Organism...

Describe the colony morphologies you observed on the MacConkey (MAC), Nutrient and Horse Blood Agar

Organism	(MacConkey) MAC	Nutrient Agar	Horse Blood Agar
E. coli
Klebsiella pneumoniae
Proteus mirabilis
Pseudomonas aeruginosa.

Expert Solution

Answer:-

A) E.coli colonies:

MacConkey- donut shaped and dark pink in color and are surrounded with dark pink area of precipitated bile salts.

Horse blood Agar- Colonies are big, circular, gray and moist. There is clearing of agar around a colony. It represents a complete breakdown of the hemoglobin of the RBC around the bacterial colony.

Nutrient Agar- They appear large, circular, low convex, grayish, white, moist, smooth and opaque.

B)Klebsiella pneumoniae:

MacConkey- colonies are large shiny and dark pink in color.

Nutrient Agar- colonies are circular, dome shaped & greyish-white.

Horse blood Agar- colonies are circular, dome shaped and greyish-white . No clearing of agar is present around the colonies , thus gamma hemolysis is present.

C) Proteus mirabilis:

MacConkey- They are non-lactose fermenting bacteria and have colorless or transparent colonies. Fishy odour present.

Horse blood Agar- Swarming effect on blood agar plate due to active motility of bacteria.

D) Pseudomonas aeruginosa:

MacConkey- It produces clear colonies on MacConkey agar as it is non-lactose fermenting bacteria.

Nutrient Agar- colonies produces greenish colour due to production of pyoverdin.

Horse blood Agar- Colonies are surroundend by a wide zone of beta-hemolysis.

gladiator answered 3 years ago

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