You are asked by your new boss to prepare 750 ml of a 0.5M pH 8.4...

You are asked by your new boss to prepare 750 ml of a 0.5M pH 8.4 tris- (hydroxymethyl) amminomethane (Tris) buffer. The Tris base is provided in the solid form (mol. wt. 121.1 g/mole, pKa 8.1). You are also supplied with solutions of 0.5 M HCl and 0.5M NaOH. Explain how you would prepare this buffer.

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queen_honey_blossom answered 2 weeks ago

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