(a) DCPIP was used to detect the activity of Succinate Dehydrogenase (SDH). Please explain the principle...

(a) DCPIP was used to detect the activity of Succinate Dehydrogenase (SDH). Please explain the principle of this detection method.

(b) Auto-oxidation is the spontaneous oxidation of a compound in the presence of oxygen. DCPIP undergoes auto-oxidation during the experiment, which may result in inaccurate results. Please highlight the appropriate items in the list below (a wrong choice will lead to a negative mark) and revise the current experimental protocol. You should clearly explain the reason(s) of choosing each item from the list and explain the rationale behind your suggested revision to the protocol. Please answer in point form with the aid of graphs/flowcharts.

10mM succinate
50M succinate
0.23 mg/ml DCPIP
200M KCN
2mM KCN
10mM CaCl₂
100M CaCl₂
Hypotonic assay buffer
Osmotic assay buffer
Measuring tape
Coffee
Acetylcholine
Argon gas (pressurized)

Ring stand and accessories
Syringe needle
Rubber tube
Cuvette
Flame photometer
Spectrophotometer
Faraday cage
Glass hooks
Metal probe
Scissors
Aluminum foil
Silicone rubber cap for cuvette
Knife

Expert Solution

gladiator answered 2 years ago

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