Question

In: Biology

(a) DCPIP was used to detect the activity of Succinate Dehydrogenase (SDH). Please explain the principle...

(a) DCPIP was used to detect the activity of Succinate Dehydrogenase (SDH). Please explain the principle of this detection method.

(b) Auto-oxidation is the spontaneous oxidation of a compound in the presence of oxygen. DCPIP undergoes auto-oxidation during the experiment, which may result in inaccurate results. Please highlight the appropriate items in the list below (a wrong choice will lead to a negative mark) and revise the current experimental protocol. You should clearly explain the reason(s) of choosing each item from the list and explain the rationale behind your suggested revision to the protocol. Please answer in point form with the aid of graphs/flowcharts.

  • 10mM succinate
  • 50M succinate
  • 0.23 mg/ml DCPIP
  • 200M KCN
  • 2mM KCN
  • 10mM CaCl2
  • 100M CaCl2
  • Hypotonic assay buffer
  • Osmotic assay buffer
  • Measuring tape
  • Coffee
  • Acetylcholine
  • Argon gas (pressurized)
  • Ring stand and accessories
  • Syringe needle
  • Rubber tube
  • Cuvette
  • Flame photometer
  • Spectrophotometer
  • Faraday cage
  • Glass hooks
  • Metal probe
  • Scissors
  • Aluminum foil
  • Silicone rubber cap for cuvette
  • Knife

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