You prepare 50 mL of 50 mM HEPES buffer at pH 7.4. Indicate the amounts of...

You prepare 50 mL of 50 mM HEPES buffer at pH 7.4. Indicate the amounts of each of the following reagents you would use (if applicable)?

HEPES ______(g); 10 M NaOH______(mL); 1 M HCl________(μL)

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queen_honey_blossom answered 6 months ago

pka is 7.21, Assigned pH: 6.8______ . prepare 50 mL of a 50 mM buffer at...

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if you need to prepare a buffer solution at pH 7.4 from a weak base/ conjugate...

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Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of 0.54 M HCl to 59 mL of the buffer. What is the new pH of the solution? (Enter your answer to three significant figures.) Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of 0.54 M NaOH to 59 mL of the buffer. What is the new pH of the solution (Enter your answer to three significant figures.)

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of...

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Describe in detail how you would preapre 100 mL of 0.15 M HEPES buffer at pH...

Describe in detail how you would preapre 100 mL of 0.15 M HEPES buffer at pH 7.30 at room temperature. The pKa of HEPES is 7.55. Prepare the buffer by using the free acid form, and then titrate with a 1.00 M strong acid/base until the desired pH is reached Hints: - Calculate the amount of HEPES free acid that you need to prepare 100 mL of 0.100 M solution using the molecular weight of 238.3 g/mol - Use the...

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You prepare 50 mL of 50 mM HEPES buffer at pH 7.4. Indicate the amounts of...

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