You prepare 50 mL of 50 mM HEPES buffer at pH 7.4. Indicate the amounts of...

You prepare 50 mL of 50 mM HEPES buffer at pH 7.4. Indicate the amounts of each of the following reagents you would use (if applicable)?

HEPES ______(g); 10 M NaOH______(mL); 1 M HCl________(μL)

Expert Solution

queen_honey_blossom answered 8 months ago

pka is 7.21, Assigned pH: 6.8______ . prepare 50 mL of a 50 mM buffer at...

pka is 7.21, Assigned pH: ____6.8__________ . prepare 50 mL of a 50 mM buffer at your assigned pH, calculate the ratios of the monobasic and dibasic species you will be using with the Henderson-Hasselbalch equation. Ratios: Monobasic species: ______________ Dibasic species: ______________ Volumes: Monobasic species: ______________ Dibasic species: ______________

How would you make up 100 ml of 50 mM Tris-HCl buffer at pH 7.4, using...

How would you make up 100 ml of 50 mM Tris-HCl buffer at pH 7.4, using the 0.5 M Tris base stock solution, and any other required materials?

Calculate the amount (in millimoles) of protonated and unprotonated HEPES in 25 mL of 50 mM HEPES at pH 7.7 (the pKa of HEPES is 7.47)

Part 1 Calculate the amount (in millimoles) of protonated and unprotonated HEPES in 25 mL of 50 mM HEPES at pH 7.7 (the pKa of HEPES is 7.47) Part 2 To adjust the pH of the HEPES solution in problem 7, 1mL of 50mM NaOH was added. Calculate the amount (in millimoles) of protonated and unprotonated HEPES. What is the pH after the addition of NaOH?

If you have 120. mL of a 0.100 M HEPES buffer at pH 7.55 and you...

If you have 120. mL of a 0.100 M HEPES buffer at pH 7.55 and you add 2.00 mL of 1.00 M HCl, what will be the new pH? (The pKa of HEPES is 7.55.) pH =

You must make a sodium phosphate buffer (500 ml at 50 mM) at a pH value...

You must make a sodium phosphate buffer (500 ml at 50 mM) at a pH value of 6.8. Assume that the pKa is 7.0. MW oh NaH2PO4 = 138g/mol. MW of NaH2PO4 = 268 g/mol Show the weak acid base reaction with proper structures. use the Henderson/ Hasselbalch relationship to obtain molarity of each species. Calculate the grams of each salt required.

You have 200 ml of 0.1M HEPES buffer, pH 7.5. You add 2 ml of 0.4M...

You have 200 ml of 0.1M HEPES buffer, pH 7.5. You add 2 ml of 0.4M HCl. The pKa of HEPES is 7.5. a) what will the resulting pH be? b) what would the pH be if no HEPES were present (that is, you mixed water and HCl)?

if you need to prepare a buffer solution at pH 7.4 from a weak base/ conjugate...

if you need to prepare a buffer solution at pH 7.4 from a weak base/ conjugate acid buffer system which buffer system would you pick and why?

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of...

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of 0.54 M HCl to 59 mL of the buffer. What is the new pH of the solution? (Enter your answer to three significant figures.) Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of 0.54 M NaOH to 59 mL of the buffer. What is the new pH of the solution (Enter your answer to three significant figures.)

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of...

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of 0.45 M HCl to 51 mL of the buffer. What is the new pH of the solution?

Describe in detail how you would preapre 100 mL of 0.15 M HEPES buffer at pH...

Describe in detail how you would preapre 100 mL of 0.15 M HEPES buffer at pH 7.30 at room temperature. The pKa of HEPES is 7.55. Prepare the buffer by using the free acid form, and then titrate with a 1.00 M strong acid/base until the desired pH is reached Hints: - Calculate the amount of HEPES free acid that you need to prepare 100 mL of 0.100 M solution using the molecular weight of 238.3 g/mol - Use the...

Question

You prepare 50 mL of 50 mM HEPES buffer at pH 7.4. Indicate the amounts of...

Solutions

Expert Solution

Related Solutions

pka is 7.21, Assigned pH: 6.8______ . prepare 50 mL of a 50 mM buffer at...

How would you make up 100 ml of 50 mM Tris-HCl buffer at pH 7.4, using...

Calculate the amount (in millimoles) of protonated and unprotonated HEPES in 25 mL of 50 mM HEPES at pH 7.7 (the pKa of HEPES is 7.47)

If you have 120. mL of a 0.100 M HEPES buffer at pH 7.55 and you...

You must make a sodium phosphate buffer (500 ml at 50 mM) at a pH value...

You have 200 ml of 0.1M HEPES buffer, pH 7.5. You add 2 ml of 0.4M...

if you need to prepare a buffer solution at pH 7.4 from a weak base/ conjugate...

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of...

Using a 0.20 M phosphate buffer with a pH of 7.4, you add 0.71 mL of...

Describe in detail how you would preapre 100 mL of 0.15 M HEPES buffer at pH...