1. The following gRNA has been designed: ATGAACGAAATAGA If we were to use this gRNA sequence...

1. The following gRNA has been designed: ATGAACGAAATAGA

If we were to use this gRNA sequence in a genome editing project, which of the following genomes will be edited with off-target effects, explain why and how many off-target effects would be observed? (2pts)

Budding yeast - Saccharomyces cerevisiae: 12x10^6 bp

Zebrafish - Danio rerio: 1.61x1^9 bp

Round worm - Caenorhabditis elegans: 9700x10^4 bp

Arabidopsis - Arabidopsis thaliana: 157,000,000 bp

Expert Solution

Answer:

Please Rate My Answer....... Thank.....u...

gladiator answered 11 months ago

The sequence of numbers 0.85, 0.97, 0.48, 0.62 and 0.60 has been generated. a- Use the...

The sequence of numbers 0.85, 0.97, 0.48, 0.62 and 0.60 has been generated. a- Use the Kolmogorov - Smirnov test with α = 0.05 to determine if the hypothesis that the numbers are uniformly distributed on the interval [0, 1] can be rejected ? b- Would your conclusion change if we had 0.59 instead of the middle random number 0.48?Why?

Create ER diagram for the following: A database has been designed for a Human Resources for...

Create ER diagram for the following: A database has been designed for a Human Resources for a school in the UK. The database includes records of the teachers and their holidays. The Dean of this school has the power to approve those holidays for all teachers in the school. For each teacher, Human Resources keeps track of the Teacher's ID, name, Cell phone number(s), total number of holidays for each year, number of unemployed holiday days remaining in the...

1. For this question, assume a simple programming language (SPL) has been designed to write a...

1. For this question, assume a simple programming language (SPL) has been designed to write a straight-line program (SLP). Programs written in this simple programming language, such as the sample program P1 shown below, allow the implementation of only statements and expressions, but no loops or if-statements. Note the print function will take an expression list as its arguments. Program P1: a := 2 + 5; b := (print (a, a-1), 7 * a) ;...

1) You are a molecular biologist who has been asked to sequence a cancer patient’s nuclear...

1) You are a molecular biologist who has been asked to sequence a cancer patient’s nuclear exome. You have access to all sequencing-bysynthesis technologies on the market. Explain which method you would use for this sample and provide a summary of the approach? b) A scientific researcher has obtained 5x coverage for a whole genome using massively parallel sequencing. Giving your reasoning, explain whether they should use this sequencing data for publication in a good quality scientific journal. Multiple detailed...

Use the following to answer 1 - 6. Two separate tests are designed to measure a...

Use the following to answer 1 - 6. Two separate tests are designed to measure a student's ability to solve problems. Several students are randomly selected to take both tests and the results are given below. Test A 64 48 51 59 60 43 41 42 35 50 45 Test B 91 68 80 92 91 67 65 67 56 78 71 What is the value of the linear coefficient r? .567 .974 .863 .488 Question 2 Assuming a 0.05...

1. A taste-testing experiment has been designed so that four brands of Colombian coffee are to...

1. A taste-testing experiment has been designed so that four brands of Colombian coffee are to be rated by nine experts. To avoid any carryover effects, the tasting sequence for the four brews is randomly determined for each of the nine expert tasters until a rating on a 7-point scale (1=extremely unpleasing, 7=extremely pleasing) is given for each of the following four characteristics: taste, aroma, richness, and acidity. The following table displays the summated ratings-accumulated over all four characteristics. BRAND...

1. Assume that a bit sequence "10000000" has been transmitted through a transmission media using parity...

1. Assume that a bit sequence "10000000" has been transmitted through a transmission media using parity bits. Two bits have been altered in the path and the receiver has received it as "10000011". will the receiver be able to detect the error. Justify your answer. 2.Which of the following is NOT a consequence of poor project management? a. Time slippage. b. Loss of intellectual property. c. Failure to obtain anticipated benefits. d. Costs that exceed budgets. 3. Simon's software company...

1-The following list of readings were taken in sequence during a levelling survey: Readings (m): 1.163,...

1-The following list of readings were taken in sequence during a levelling survey: Readings (m): 1.163, 2.592, 3.616, 0.778, 2.348, 1.838, 2.469, 1.666, 2.893, 1.594 The level instrument was moved after the fifth and eighth readings. Required: (i) Fill in the leveling table and find the RLs of all points. (RL of the first observed point = 105.00m). Use R & F method and check using HCI method. Apply arithmetic check in both cases. *Please solve it by detail.

An acceptance sampling plan with and has been designed with a producer’s risk of .075. Was...

An acceptance sampling plan with and has been designed with a producer’s risk of .075. Was the value of .01, .02, .03, .04, or .05? What does this value mean? What is the consumer’s risk associated with this plan if is .25? I already know the answer to this but could you please show me how to get the answer using excel

Wisconsin Electronics has received an order for 1,000 specially designed TV/VCR combinations. The following standards were...

Wisconsin Electronics has received an order for 1,000 specially designed TV/VCR combinations. The following standards were compiled for this order: Direct materials: Part #123 $25 per TV/VCR Part #456 $50 per TV/VCR Direct labor: 2.0 hours per TV/VCR at $15 per hour Manufacturing overhead: $40 per direct labor hour Assume the company adds a 10% markup to arrive at a selling price. The bid price for this order totals: A. $203,500. B. $115,500. C. $185,000. D. $80,000.

Question