Describe how you can use PCR to obtain a gene fragment X that
can be cloned into a
plasmid vector for expression of the recombinant protein. Include
in your description
how the PCR reaction will be performed, how the amplicon will be
analyzed, the use of
restriction enzymes, how cloning will be achieved, how you will
know that you have a
recombinant containing the insert gene fragment X and how you will
detect the
recombinant protein.