Question

38. What is the purpose of heat fixation?

	A.	To facilitate the even dispersal of organisms on the slide
	B.	To alter the cell wall structure for better viewing under microscopy
	C.	To kill and adhere cells to the slide
	D.	To encourage the growth of bacteria on the slide

10. What genus of bacteria is usually differentiated by a positive urea test and a positive phenylalanine deamination test?

	A.	Escherichia
	B.	Proteus
	C.	Bacillus
	D.	Staphylococcus

39. What color is the capsule after this staining technique?

	A.	Purple
	B.	Pink
	C.	Clear
	D.	Black

2. Why is mineral oil added to the surface of the tube in this test?

	A.	The test should be conducted under aerobic conditions.
	B.	The test should be conducted under anaerobic conditions.
	C.	It is important to trap oxygen into the medium for the reaction to occur.
	D.	A & C

Answer 1

38. Answer: C. To kill and adhere cells to the side

Heat fixation is a process used in microbiological slide preparation which kills the bacterial cell (thereby breaking it open) and also improves the adherence of the cell to the slide

10. Answer: B. Proteus

Urea test and phenylalanine deamination tests are used to differentiate the genus Proteus. The bacteria belonging to this genus have the enzyme that can remove the amine group from phenylalanine, converting it to phenylpyruvic acid. Also, the bacteria of this genus have the enzyme urease and thus give positive urea test.

39. The question is incomplete. The question says " 'this' technique" but doesn't mention the name of the technique. Please mention the technique name in comments and we would be happy to provide you the answer.

2. This question is also incomplete. It says " 'this' test" but doesn't mention the name of the test. Please mention the technique name in the comments and we would provide you the answer.

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