Treatment of a polypeptide with Tris-(2-carboxyethyl)-phosphine
hydrochloride (TCEP) yields two peptides:
1. I-Q-K-H-C-R-C-A-K-M-V-S
2. F-C-R-L-K-D-C-K-N-D
Treatment of the intact polypeptide with Trypsin yields
fragments with the following amino acid composition:
(Ser, Met, Val) (Lys, Gln, Ile) (Phe, Ala, Cys2, Arg, Lys) (Asp,
Asn) (Leu, Lys)
(Lys, His, Asp, Cys2, Arg)
a) The intact (untouched) polypeptide is labeled with
1-Fluoro-2,4-dinitrobenzene (FDNB) labeling, followed by acid
hydrolysis. What will be observed?
b) Name and briefly describe a method which may determine if...