Question

Which of the following statements about the Pre Initiation Complex in eukaryotes is not correct?

The function of the Pre Initiation Complex in eukaryotes is to place and orient RNA polymerase II so that transcription can be initiated.

TF IID contains TATA binding proteins and assembles at the TATA box.

TF IID assembles at ~ -32 to -24 site along with other transcription factors.

The Pre Initiation Complex of proteins assembles at the -10 and -35 sequences.

RNA polymerase II begins to elongate when it dissociates from the PIC.

Answer 1

Eukaryotic transcription

1. The function of the Pre Initiation Complex in eukaryotes is to place and orient RNA polymerase II so that transcription can be initiated--- correct. (The function of the PIC is to correctly assemble the RNA pol II and DNA unwinding to initiate the transcription)

2.TF IID contains TATA binding proteins and assembles at the TATA box: correct- TF IID is composed of TBP (DNA bindind domain) which recognize the core promoter of eukaryotes (wihich is also known as TATA box) and assamble the other PIC factors.

3. TF IID assembles at ~ -32 to -24 site along with other transcription factors: Correct--- he TATA box is a DNA sequence (5'-TATAAA-3') within the core promoter region its located on 25-35 base pairs before the transcription start site of a gene.

4. The Pre Initiation Complex of proteins assembles at the -10 and -35 sequences: Incorrect.: -10 (10 bp 5' or upstream) and -35 positions from the transcription start site is the promoter of prokaryotes. Pribnow box (TATAAT) is located at the -10 position and -35 element is equivalent to the eukaryotic TATA box.

5. RNA polymerase II begins to elongate when it dissociates from the PIC.: correct. during elongation the PIC is no longer required.