As a class, we added several different reagents to our Winogradsky columns. We also made one...

As a class, we added several different reagents to our Winogradsky columns. We also made one control-column that had nothing added to it (aside from sediment and water). Everyone added paper towels (but at varying depths), and sulfate to the bottom of their columns. The rest of the treatments are listed below. Please indicate what types of organisms you might enrich with that type of treatment and why, or how and why that treatment might result in a different type of growth rate or metabolic rate. (Possible organisms are listed but not limited to: Oxygenic phototrophic microorganisms, Diatom, Cyanobacteria, Filamentous algae, Purple sulfur-oxidizing phototrophic bacteria, Green sulfur-oxidizing phototrophic bacteria, Aerobic heterotrophs, Fermenting bacteria, Sulfate-reducing bacteria...)

10mM Nitrate only:
100mM Nitrate only:
10mM Phosphate only:
100mM Phosphate only:
10mM Nitrate + Phosphate:
100mM Nitrate + Phosphate:
10mM Nitrate + Phosphate + 1mM DIC (bicarbonate):
100mM Nitrate + Phosphate + 6mM DIC (bicarbonate):

Expert Solution

Winogardsky column is a device that is used to culture a large diversity of microrganisms. The top most layer will be that of Clostridium spp. which will usr the cellulose as a source of energy and release CO2,which will be used up by green sulphur oxidizing phototropic bacteria followed by purple sulphur oxidizing phototropic bacteria. This is followed by sulfate reducing bacteria and fermenting bacteria.

When 10mM and 100mM Nitrate is added, thermophilic anaerobic chemolithotropic bacteria will be obtained as they can form ammonia from nitratrnitrate by oxidising Sulphur.

When 10mM Phosphate is added, Non-sulphur oxidising bacteria will be obtained which will liberate H2S

When 10mM and 100mM of Nitrate and Phosphate is added, Aerobic Halophiles will be obtained.

When 10mM nitrate+phosphate+1 mM DIC(bicarbonate) was added, aerobic halophiles as well as Proteobacteria, Firmicutes and Bacteriods will be obtained .

Similiar will be the result when 100mM nitrate+phosphate+6mM DIC( bicarbonate)is added

gladiator answered 10 months ago

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