Label T/F micro-RNA: (only one may be true) T/F: pathway uses Drosha, but not RISC or...

Label T/F micro-RNA: (only one may be true)

T/F: pathway uses Drosha, but not RISC or Dicer.
T/F: they are encoded by cellular genes.
T/F: pathway uses Drosha, RISC and Dicer
T/F: they are encoded by cellular genes and microRNA pathway uses Drosha, RISC and Dicer.
T/F: they are encoded by cellular genes and microRNA pathway uses Drosha, but not RISC or Dicer.

Expert Solution

mi RNA is the method of gene silencing which is used to inhibit the expression of mRNA
Statmement 1 is False
mi RNA pathway is the process which require the functioning of both the enzymes. DROSHA as well as DICER.

Statement 2 is False
MiRNA are non coding RNA molecules which will post transcriptionally regulate the expression of genes. It generally bind to the mRNA at its untranslated region and will inhibit or will reduce the production of protein by mRNA destabilizing.

Statement 3 is True
miRNA pathway needs the functioning of 3 enzymes
1. DICER is the enzyme which will cut the double stranded RNA into short sequences of double stranded RNA.
2. RISC(RNA Induced Silencing Complex)- it will cut the strand of mRNA.
3. DROSHA - base hairpin is cleaved by DROSHA in the form of miRNA

Statsment 4 is False
miRNA are non coding molecules they are not encoded by the cellular genes.

Statement 5 is False
miRNA needs the working of all the 3 enzymes that is DROSHA, DICER and RISC.

gladiator answered 2 years ago

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