Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by...

Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by a biological chemist using 10.0M NaOH to a pH of 7.79 using 31.52 g of (HOCH2)3CNH3Cl, or TrisH+, (MW=157.597g/mol, pKa 8.075).

(a) Calculate the ratio of [Tris]/[TrisH+] at a pH of 7.79.

(b) Calculate the initial moles of (HOCH2)3CNH3Cl (MW=157.597g/mol) that were added.

(c) Write the chemical reaction between the TrisH+ and the added NaOH.

(d) Calculate the moles of hydroxide used to produce the ratio calculated in part (a).

(e) What volume of 10.0 M NaOH is needed to prepare the buffer?

(f) Now you dilute the solution from above to 1.0 L and take half of the buffer (500.0 mL) and add 0.0100 moles solid HCl. What is the new pH?

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genius_generous answered 10 months ago

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2, often abbreviated as Tris. At 25 ∘C, Tris has a pKb of 5.91. The hydrochloride of Tris is (HOCH2)3CNH3Cl, which can be abbreviated as TrisHCl. Part A What volume of 10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using 31.52 g of TrisHCl? Express your answer in milliliters using two significant figures. 6.7 mL SubmitHintsMy AnswersGive UpReview Part...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2, often abbreviated as Tris. At 25 ?C, Tris has a pKb of 5.91. The hydrochloride of Tris is (HOCH2)3CNH3Cl, which can be abbreviated as TrisHCl. Part A What volume of 10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using 31.52 g of TrisHCl? . CORRECT6.7 mL Part B This procedure represents only one of many ways to prepare...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2, often abbreviated as Tris. At 25 ?C, Tris has a pKbof 5.91. The hydrochloride of Tris is (HOCH2)3CNH3Cl, which can be abbreviated as TrisHCl. Question 1.) What volume of 10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using 31.52 g of TrisHCl? ANSWER IS 6.67 Question 2.) The buffer from Part A is diluted to 1.00 L. To...

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Tris, (HOCH2)3CNH2 (pKb = 5.91), a very common buffer for studying biochemical processes, is prepared by...

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In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...

In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...