Question

What is CreERT2? Give an example of how it can be used

Answer 1

CreERT2 encodes a Cre recombinase (Cre)  amalgamate to a mutant sex hormone ligand-binding domain (ERT2) that needs the presence of antagonist for activity.

Example- Tamoxifen-inducible NaV1.8-CreERT2 recombinase activity in sensitive neurons of dorsal root ganglia.

To explore the perform of genes expressed in adult mouse sensitive neurons, we have a tendency to generated heterozygous knock-in mice expressing the tamoxifen-inducible Cre recombinase construct CreERT2 downstream of the Na(V)1.8 promoter. CreERT2 encodes a Cre recombinase (Cre) consolidated to a mutant steroid ligand-binding domain (ERT2) that needs the presence of estrogen antagonist for activity. we've got antecedently shown that heterozygous Na(V)1.8-Cre mice can delete loxP flanked genes specifically in sensitive sensory neurons from embryonic day fourteen. we have a tendency to thus used identical strategy of homologous recombination and mouse generation, work the Cre container with CreERT2. No practical Cre recombinase activity was found in CreERT2 mice crossed with newsman mice within the absence of estrogen antagonist. we have a tendency to found that, like Na(V)1.8-Cre mice, practical Cre recombinase was gift in sensitive sensory neurons when estrogen antagonist induction in vivo. However, the proportion of dorsal root neural structure (DRG) neurons expressing practical Cre activity was abundant reduced (<10% of the amount found within the Na(V)1.8-Cre mouse). we have a tendency to additionally examined Cre recombinase activity in sensory neurons in culture. when treatment with one muM estrogen antagonist for forty eight h, V-day of DRG neurons showed Cre activity. Na(V)1.8-CreERT2 animals could so be helpful for single cell studies of the practical consequences of sequence ablation in culture, however ar unlikely to be helpful for activity studies.