Question

After you synthesize a molecule, you are asked to determine your product’s melting point and mass, collect its IR spectrum, and analyze it by thin layer chromatography. Which of these experiments gives you information on the purity of the product.

Answer 1

After you synthesize a molecule, you are asked to determine your product’s melting point and mass, collect its IR spectrum, and analyze it by thin layer chromatography. Which of these experiments gives you information on the purity of the product.

IR spectroscopy:

1. We using IR spectroscopy, Identification of functional group and structural elucidation of unknown molecules,
2. We using IR spectroscopy, can able to study of covalent bonds in molecule,
3. We using IR spectroscopy, can able to determine impurities in a compound,
4. We using IR spectroscopy, find Ratio of Cis-Trans isomers in a mixture of compounds,
5. We Using IR spectroscopy, can able to study the presence of water in our sample.

Thin Layer Chromatography:

1. We using Thin-Layer Chromatography, Identifying Isolated Molecule,
2. We using Thin-Layer Chromatography, Separating Components of Extract,
3. We using Thin-Layer Chromatography, Determining their Purity,
4. We using Thin-Layer Chromatography, To monitor the progress of a Reaction.

Melting Point:

1. The physical properties of a substance can be used to establish its purity, These property include the melting point and boiling point.
2. Different molecule tend to have different melting and boiling points,
3. The pure the compound, the higher the melting point,
4. The pure the compound, the narrower the melting point Range.

Conclusion:

From Thin-Layer chromatography we get Purity of molecule, (but we need to reference standard) at the same time we conform that molecule using melting point of the particular molecule.