Suppose that you are given the task of determining the SCN- content of a blood sample...

Suppose that you are given the task of determining the SCN^- content of a blood sample by forming the Fe(SCN)²⁺ complex and measuring the absorbance. You begin by taking a 10.00-mL volume of the blood sample, centrifuging it, transferring 0.50 mL of the clear serum to a flask, and then adding 15.00 mL of 0.20M Fe³⁺. The %T of this solution, measured at 460 nm in a 1.0-cm diameter cuvet, is 75%. A calibration curve, prepared from Fe(SCN)²⁺ standards, indicates that the Fe(SCN)²⁺ concentration of the diluted sample solution is 0.033 mM. Calculate the SCN^- content (in mM) of the original blood sample.

(Atomic weight of Fe = 55.85 g/m; formula weight of SCN^- = 58.08 g/m)

A) 0.033 mM

B) 0.33 mM

C)0.40 mM

D) 0.82 mM

E) 1.02 mM

Expert Solution

queen_honey_blossom answered 11 months ago

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