Enzyme catalysis catalyzes the breakdown of H2O2. Data are: [H2O2] (mol/L) 0.001 0.002 0.005 inital velocity...

Enzyme catalysis catalyzes the breakdown of H2O2. Data are:

[H₂O₂] (mol/L)	0.001	0.002	0.005
inital velocity (mol/L.s)	1.38x10^-3	2.67x10^-3	6.00x10^-3

If the concentration of the enzyme is 4.0x10- 9 mol / L, plot the data from the data and calculate the Michaelis constant and turnover number to find the maximum starting speed?

Expert Solution

Vmax (maximum initial velocity)

Km (Michaelis Menten constant)

Turnover Number

.

Note : Your feedback is much appreciated. Give a 'Thumbs Up' if you liked and comment if you still have some doubts.

.

Explanation :

S (mol/L)	Vo (mol/L.s)	1/S (L/mol)	1/Vo (s.L/mol)
0.001	0.00138	1000.00	724.64
0.002	0.00267	500.00	374.53
0.005	0.00600	200.00	166.67

.

Lineweaver-Burk Plot

From graph,

Intercept = Vmax = 26.557 mol/L.s

Slope = Km / Vmax

Km = slope * Vmax = 0.6977 * 26.557 = 18.53 mol/L

Turnover Number = Vmax / Enzyme concentration

.

Note : Your feedback is much appreciated. Give a 'Thumbs Up' if you liked and comment if you still have some doubts.

queen_honey_blossom answered 3 years ago

Calculate the ionic strength in a solution that contains 0.010 mol KCl, 0.005 mol MgCl2, 0.002...

Calculate the ionic strength in a solution that contains 0.010 mol KCl, 0.005 mol MgCl2, 0.002 mol MgSO4, and 100g H2O.

The enzyme fumarase catalyzes the hydrolysis of fumarate: Fumarate(aq) + H2O(liq) → L-maleate(aq) The turnover number...

The enzyme fumarase catalyzes the hydrolysis of fumarate: Fumarate(aq) + H2O(liq) → L-maleate(aq) The turnover number k2 for this enzyme is 2.5 × 103 s-1, and the Michaelis constant is 4.2 × 10-6 mol / L. (a) What is the rate of fumarate conversion v if the total concentration of the enzyme is 1.0 × 10-7 mol / Land the concentration of fumarate is 3.0 × 10-4 mol / L? (b) What is the ratio (v/vmax) in the conditions of...

Calculated R Value (L-atm/mol - K) Standard Deviation: Group 1 0.1021 0.001 Group 2 0.0626 0.012...

Calculated R Value (L-atm/mol - K) Standard Deviation: Group 1 0.1021 0.001 Group 2 0.0626 0.012 Group 3 0.0831 0.010 Group 4 0.0811 0.020 1. Provide a brief explanation of accuracy and precision. Be sure you use your own words. 2. Which group was the most accurate? Explain your reasoning. 3. Which group had the best precision? Explain your reasoning. 4. Which group had the most consistent experimental results from the three trials? Explain your reasoning. 5. One of these...

The following data were collected for the reaction 2 H2O2(aq) → 2 H2O(l) + O2(g). Graph...

The following data were collected for the reaction 2 H2O2(aq) → 2 H2O(l) + O2(g). Graph the [A] vs. time, ln[A] vs time and 1/[A] vs. time. What is the rate law and rate constant of the reaction? Determine what time concentration of H2O2 will be 5.00 M? Time, sec [H 2 O 2 ], M 0 10.00 500 8.05 1000 6.88 1500 5.63 2000 4.68

Initial concentration= 1mol/L, velocity of flow=2000L/min, liquid phase reactant enter the reactor. (-rA=kCA^2 , k=5000L/mol*min) We...

Initial concentration= 1mol/L, velocity of flow=2000L/min, liquid phase reactant enter the reactor. (-rA=kCA^2 , k=5000L/mol*min) We want to achieve a conversion of 90 % of the reactant. 1) Find the volume of the reactor when using the CSTR reactor. And find the volume of the reactor when using the PFR reactor. 2) Find the conversion of A at the exit of the reactor when you make the volume of the PFR reactor equal to that of the CSTR reactor saved...

Question