2. You are given a pure protein sample to characterize and provided the following information: Its...

2. You are given a pure protein sample to characterize and provided the following information:

Its molar extinction coefficient, ε280, is 0.30 liters micromole-1 cm-1

Its ΔGo for unfolding is 1.5 kcal/mol at 37o (where RT = 0.59 kcal/mole)

A) Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 25- fold dilution of your pure protein in solution and find A280 = 0.45. What is the original concentration of the protein before dilution?

B) What is the concentration of the unfolded form of the protein in your sample?

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Expert Solution

queen_honey_blossom answered 10 months ago

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