Using a cation exchange resin, a mixture of four amino acids, Asp (pKa 3.9), Arg (pKa...

Using a cation exchange resin, a mixture of four amino acids, Asp (pKa 3.9), Arg (pKa 12.5), Ser (pKa 13) and Lys(pKa 10.5) are separated using an elution gradient of increasing NaCl solution. What would be the correct elution sequence? Explain your reasoning. Draw the chromatogram would look like for this mixture if there was twice as much Asp and Arg in the sample than Ser and Lys.

Solutions

Expert Solution

queen_honey_blossom answered 1 year ago

Next > < Previous

Related Solutions

An anion-exchange resin can be used instead of the cation-exchange resin in this experiment to separate...

An anion-exchange resin can be used instead of the cation-exchange resin in this experiment to separate the three amino acids in the known mixture. What changes to experimental protocol are required to achieve complete resolution? Support your response with an explanation. (HINT: consider pH). You have been provided a mixture containing four amino acids (Glu, His, Pro and Lys) to be separated by ion-exchange chromatography using a Dowex 50 cation-exchange, prepared in a loading buffer at pH 2.0. The following...

during cation exchange chromatography, what is the pattern in which these amino acids would be eluted?...

during cation exchange chromatography, what is the pattern in which these amino acids would be eluted? please explain the reason behind the elution pattern. amino acids are leucine, aspartic acid, alanine, lysine and phenylalanine.

. In what order will the following amino acids elute from a Dowex-50 cation exchange column...

. In what order will the following amino acids elute from a Dowex-50 cation exchange column at pH 3.2: alanine (pI = 6.02); arginine (pI = 10.76); glutamate (pI = 3.22); serine (pI = 5.68); and tryptophan (pI = 5.88)? (Ignore the polarity characteristics of the side-chains).   Wouldnt the basic ones bind and the acidic one (glu) elute off first??

You have a mixture of proteins with the following properties, using a cation exchange column, what...

You have a mixture of proteins with the following properties, using a cation exchange column, what would be the order of elution for the following mixture of proteins MW pHI A. 85,000 7.0 B. 65,000 6.2 C. 35,000 9.5

You have been provided a mixture containing four amino acids (Glu, His, Pro and Lys) to...

You have been provided a mixture containing four amino acids (Glu, His, Pro and Lys) to be separated by ion-exchange chromatography using a Dowex 50 cation-exchange, prepared in a loading buffer at pH 2.0. The following solutions are made available: The amino acid mixture in 0.04M citrate buffer at pH 2.0. 0.04M citrate buffer, pH 4.5 0.04M citrate buffer, pH 8.0 0.04M citrate buffer, pH 10.5 Is complete resolution of all amino acids achieved if the buffers are allowed to...

Biefly describe the roles of three amino acids, Asp, His, and Ser in the Chymotrypsin-catalyzed reaction.

how do buffer ph/pKa/pI values for amino acids affect their elution order during ion exchange chromatography?...

how do buffer ph/pKa/pI values for amino acids affect their elution order during ion exchange chromatography? what molecular properties affect the migration distance (on the chromatography paper) of amino acids during IEC?

All amino acids have two ionizable functional groups: an α-amino group (average pKa of 9.4) and...

All amino acids have two ionizable functional groups: an α-amino group (average pKa of 9.4) and an α-carboxylic acid group (average pKa of 2.2). Arginine also has an ionizable side chain (R group) with a pKa of ~12.5. One of the possible ionization states of arginine is shown below. At what pH would the above structure be the predominant ionization state? Note: Consider the ionization state of all three of the functional groups. The protonated form of the R group...

Determine the sequence of the following pentapeptide using the following laboratory results Asp, Arg, Gln, Ala,...

Determine the sequence of the following pentapeptide using the following laboratory results Asp, Arg, Gln, Ala, Tyr Edman Degradation gives Gln Carboxypeptidase gives Ala Trypsin gives Ala by itself Chymotrypsin gives a dipeptide with Gln

Explain how to use an ion exchange column containing carboxymethyl resin to separate a protein mixture...

Explain how to use an ion exchange column containing carboxymethyl resin to separate a protein mixture containing insulin (pl = 5.4), hemoglobin (pl = 7.1) and cytochrome c (pl = 10.6).

Subjects