Using a cation exchange resin, a mixture of four amino acids, Asp (pKa 3.9), Arg (pKa...

Using a cation exchange resin, a mixture of four amino acids, Asp (pKa 3.9), Arg (pKa 12.5), Ser (pKa 13) and Lys(pKa 10.5) are separated using an elution gradient of increasing NaCl solution. What would be the correct elution sequence? Explain your reasoning. Draw the chromatogram would look like for this mixture if there was twice as much Asp and Arg in the sample than Ser and Lys.

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queen_honey_blossom answered 2 years ago

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