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In: Chemistry

TLC Experiment Discuss the reason behind different Rf values of dye by considering the chemical structures...

TLC Experiment

Discuss the reason behind different Rf values of dye by considering the chemical structures of dyes,mobile, and stationary phase

Amaranth O- water Rf=0.9555
Amaranth O- ethanol Rf=0.6455
Amaranth O- silica gel (discuss only interactions between them)

Solutions

Expert Solution

2. Mobile phase : There are two types of mobile phases (a) Polar & (b) Non-polar. These are also help to elute substance faster. A polar mobile phase elute polar substance faster and non polar to non polar as

3. Stationary phase:

Their dispersive, ionic and polar forces contribute to increase or decrease retardation factor (Rf)


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