Determine the CFU/mL in the original suspension of E. coli given that: a) 2300 and 1990...

Determine the CFU/mL in the original suspension of E. coli given that:

a) 2300 and 1990 colonies were present when 0.1mL of the 10^-3 dilution was plated in duplicate,

b) 218 & 196 colonies were present when 0.1 mL of the 10^-4 dilution was plated in duplicate, and

c) 18 & 10 colonies were present when 0.1 mL of the 10^-5 dilution was plated in duplicate.

Solutions

Expert Solution

If any questions, please comment below. Thank You.

gladiator answered 1 year ago

Next > < Previous

Related Solutions

A grad student has a culture of E. coli with a total volume of 600 mL,...

A grad student has a culture of E. coli with a total volume of 600 mL, and OD reading at 550 nm of 1.7. He makes serial dilutions of the culture, starting with a transfer of 1 mL culture into 9 mL tryptone broth. Next, he transfers 0.1 mL of this dilution into 0.9 mL tryptone broth, and repeats this two more times to make a total of 4 serial dilutions. He then pipets 200 uL from the fourth dilution...

Given a recent outbreak of illness caused by E. coli bacteria, the mayor in a large...

Given a recent outbreak of illness caused by E. coli bacteria, the mayor in a large city is concerned that some of his restaurant inspectors are not consistent with their evaluations of a restaurant’s cleanliness. In order to investigate this possibility, the mayor has five restaurant inspectors grade (scale of 0 to 100) the cleanliness of three restaurants. The results are shown in the accompanying table. (You may find it useful to reference the q table.) Inspector Restaurant 1 2...

You have an E. coli culture of which you are trying to determine the cell concentration....

You have an E. coli culture of which you are trying to determine the cell concentration. You need to perform a serial dilution. You take 100 µL of the undiluted stock culture, add it to 9.9 mL water in tube A, and mix. Then you take 100 µL of the material in tube A, add it to 9.9 mL water in tube B, and mix. Then you take 100 µL of the material in tube B, add it to 9.9...

Which of the following statements is TRUE? E. coli sigma factors all recognize one E. coli...

Which of the following statements is TRUE? E. coli sigma factors all recognize one E. coli gene Prokaryotic mRNAs contain sequences for sigma factor binding mRNA in both E. coli and humans are translated with the same translation machinery E. coli sigma factors all bind to the core RNA polymerase mRNA always contains the nucleotide sequence encoding just one protein

separation processes for bioproducts from E. coli . Recombinant protein production from E. coli resulted in...

separation processes for bioproducts from E. coli . Recombinant protein production from E. coli resulted in the first products from biotechnology. (a) List the primary structures and components of E. coli that must be removed from a fermentation broth to purify a heterologous protein product (one that differs from any protein normally found in the organism in question) expressed for pharmaceutical use. (b) Identify a sequence of steps to purify a conjugate heterologous protein (a compound comprised of a protein...

EXPLAIN the immune system response in the following scenario with the given components: 1. E. coli...

EXPLAIN the immune system response in the following scenario with the given components: 1. E. coli found inside a poorly cooked hamburger patty. Remember, E. coli has the capability to produce toxins and is Gram-negative therefore has endotoxin present as well. Components: Mucous, mucous membranes, gastric juices, T helper cells, cytotoxic T cells, B cells, antibodies, cytokines, interferons, macrophages and dendritic cells, neutrophils, complement

The bacterium Escherichia coli (or E. coli) is a single-celledorganism that lives in the gut...

The bacterium Escherichia coli (or E. coli) is a single-celled organism that lives in the gut of healthy humans and animals. When grown in a uniform medium rich in salts and amino acids, these bacteria swim along zig-zag paths at a constant speed of 20 μm/s. The figure shows the trajectory of an E. coli as it moves from point A to point E. Each segment of the motion can be identified by two letters, such as segment BC. For the...

a) Describe how you could determine quantitate (determine how many) E. coli cells have been transformed?

a) Describe how you could determine quantitate (determine how many) E. coli cells have been transformed? b) Briefly outline your experiment. You will need a control, or standard, to analyze your experimental results, make sure to indicate this in your answer – explain what the control/standard is and how you use it.

Briefly describe the Escherichia coli (E. coli) lac operon and its regulation.

1. The concentration of particles in a suspension is 49 per mL. A 5 mL volume...

1. The concentration of particles in a suspension is 49 per mL. A 5 mL volume of the suspension is withdrawn. What is the probability that the number of particles withdrawn will be between 235 and 265? 2. a sample of 100 steel wires the average breaking strength is 46 kN, with a standard deviation of 2 kN. Find a 95% confidence interval for the mean breaking strength of this type of wire. Round the answers to three decimal places....

Subjects