Question

Compare groups of small RNAs (snRNAs; RNAi; miRNA; siRNA; piRNA)

RISC pathway and RNAs that use it

Answer 1

•The RNA-induced silencing complex, or RISC, is a multiprotein complex, specifically a ribonucleoprotein, which incorporates one strand of a single-stranded RNA (ssRNA) fragment, such as microRNA (miRNA), or double-stranded small interfering RNA (siRNA).

1. snRNAs :- Small nuclear RNA (snRNA) is a class of small RNA molecules that are found within the splicing speckles and Cajal bodies of the cell nucleus in eukaryotic.

2. RNAi :- RNA interference (RNAi) it is a key process in gene silencing

   •It inhibit gene expression or translation, by neutralizing targeted mRNA molecules.

3. miRNA:- microRNA (miRNA), Single stand RNA , acts as a template for RISC to recognize complementary messenger RNA (mRNA) transcript.

4. siRNA :- small interfering RNA (siRNA), Double strand RNA , central to RNA interference- process in gene silencing .

5. •piRNA :- Piwi-interacting RNA (piRNA) is the largest class of small non-coding RNA molecules

   •involved in the epigenetic and post-transcriptional silencing of transposable elements .