How could you use antibodies to detect the presence of a specific cell type in a...

How could you use antibodies to detect the presence of a specific cell type in a tissue sample? Is there a specific name for this process?

Expert Solution

The name of this process is - IMMUNOHISTOCHEMISTRY.

This is a technique for identifying cellular or tissue constituents ( antigen) by means of antigen antibody interactions, the site of antibody binding being identified either by direct labeling of the antibody or by use of a secondary labeling method.

The principle of this method is -

Immunohistochemistry is a method for localizing specific antigen in tissues or cells based on antigen antibody reaction.

Fluorescent dye , colloidal metal , hapten, radioactive marker is used in this method.

The associated antibodies and antigens are held together by a combination of hydrogen bonds , electrostatic interaction and van der wal's forces.

STEPS-

sample preparation>fixation> tissue sectioning> paraffin embadding > inactivation and blocking> antigen retrival> detection>counterstain.

APPLICATION- cancer detection, treatment of cancer, research purpose, different diagnosis.

gladiator answered 2 years ago

Design an indirect IF protocol that could be used to detect the presence of antibodies to...

Design an indirect IF protocol that could be used to detect the presence of antibodies to SARS CoV-2, the virus causing covid-19. Assume that the specificity of the antibodies from patients would be against epitopes on the spike proteins of the virus. Include both a description of the protocol and a diagram showing the steps of your protocol. (Draw the diagram, take a picture of it, and then insert that JPEG image into this word document).

Describe an ELISA test to detect the presence of HIV antibodies in a patient.

How is it that antibodies can only detect one antigen and how is this useful in...

How is it that antibodies can only detect one antigen and how is this useful in research?

Using a coil of wire and an oscilloscope, explain how you can detect the presence of...

Using a coil of wire and an oscilloscope, explain how you can detect the presence of the Earth's magnetic field. Using a coil of wire and an oscilloscope, explain how you could show that a magnet is producing a magnetic field. A series LRC circuit is driven at a frequency which is much less than the resonant frequency with a sine wave voltage source V0sin(wt). What is the approximate voltage across the capacitor? Briefly explain A series LRC circuit is...

1. Antibodies against both type A and type B red blood cell antigens are found in...

1. Antibodies against both type A and type B red blood cell antigens are found in the plasma of a person whose blood is: a)any of these types b)type O c)type A d)type AB e)type B 2.What plasma protein helps draw water from tissues into capillaries to maintain blood volume and pressure? a)albumin b)gamma globulins c)fibrinogen d)beta globulins e)alpha globulins 3) Fibrinogen is to fibrin as a)amino acids are to proteins b)deoxyribonucleotides are to DNA c)All of these choices d)ribonucleotides...

Question

How could you use antibodies to detect the presence of a specific cell type in a...

Solutions

Expert Solution

Related Solutions

Design an indirect IF protocol that could be used to detect the presence of antibodies to...

Describe an ELISA test to detect the presence of HIV antibodies in a patient.

How is it that antibodies can only detect one antigen and how is this useful in...

Using a coil of wire and an oscilloscope, explain how you can detect the presence of...

1. Antibodies against both type A and type B red blood cell antigens are found in...

why, in an immunostaining procedure for a specific antigen, do you normally use two antibodies sequentially?...

How could you use work engagement theory to combat burnout? Be specific and share examples.

How could you use play in light of Vygotsky’s sociocultural theory? How could you use play...

how to use an antibody to probe or detect a protein.

How does the body manufacture antibodies for a specific pathogen? How do they recognize which ones...