How could you use antibodies to detect the presence of a specific cell type in a...

How could you use antibodies to detect the presence of a specific cell type in a tissue sample? Is there a specific name for this process?

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Expert Solution

The name of this process is - IMMUNOHISTOCHEMISTRY.

This is a technique for identifying cellular or tissue constituents ( antigen) by means of antigen antibody interactions, the site of antibody binding being identified either by direct labeling of the antibody or by use of a secondary labeling method.

The principle of this method is -

Immunohistochemistry is a method for localizing specific antigen in tissues or cells based on antigen antibody reaction.

Fluorescent dye , colloidal metal , hapten, radioactive marker is used in this method.

The associated antibodies and antigens are held together by a combination of hydrogen bonds , electrostatic interaction and van der wal's forces.

STEPS-

sample preparation>fixation> tissue sectioning> paraffin embadding > inactivation and blocking> antigen retrival> detection>counterstain.

APPLICATION- cancer detection, treatment of cancer, research purpose, different diagnosis.

gladiator answered 1 year ago

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How could you use antibodies to detect the presence of a specific cell type in a...

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