describe a real life application of the plaque assay

Expert Solution

The plaque assay is a very well-established tool for evaluating concentration of viruses as it relates to the dose of infection. This assay is focused on calculating the number of pfu (plaque-forming units) produced within a virus-infected cell monolayer. Once a virus-infected cell lysis, plaques form, leading to a subsequent process of neighboring cell infection and lysis. PRNT (Plaque Reduction Neutralization) assay is a powerful twist on the plaque assay used to test antibody nutrilizarion.

Plaque-based assays are the typical approach used to assess infectious dose concentration of the virus.

Utility of plaque assay is to phenotypic characterization of a genetically modified Plasmodium falciparum clone known to exhibit a poorly defined growth defect.

Plaque assay also used by testing separate plaques in cell culture for the direct quantification of infectious antiviral substances and virons.

gladiator answered 1 year ago

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