Question

The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions.

-SDS-PAGE utilizes agarose gel to separate proteins.

-smaller proteins migrate faster through the polyacrylamide gel.

-Protein-SDS complexes migrate toward the positive electrode.

-Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size.

-Proteins are visualized using a dye that binds to the gel matrix, but not to proteins.

-A protein binds roughly 1.4 times its mass of SDS resulting in a large overall negative charge.

Answer 1

Hey there, here are your answers-

The true statements are-

smaller proteins migrate faster through the polyacrylamide gel.

A protein binds roughly 1.4 times its mass of SDS resulting in a large overall negative charge.

The SDS and mercaptoethanol reduce any disulfide bridges in a protein and SDS denatures protein so that the protein opens up into linear structure with SDS bound to it so that it has a negatively bound SDS and it can move towards the positive electrode.

The separation is purely based on size, smaller proteins move faster the polyacrylamide gel.