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Justin want to study the motility of Treponema. He selected the transmission electron microscope. This is...

Justin want to study the motility of Treponema. He selected the transmission electron microscope. This is the _____ choice because ______.

A. Right, motility is easily observed wit the TEM

B. Right, you can see the flagella with the TEM

C. Wrong, greater magnification needed

D. Wrong, all cells viewed in the TEM will be dead

Solutions

Expert Solution

Ans.

The answer is (D) Wrong,all Cells viewed in the TEM will be dead.

Explanation:

Eventhough the resolution of image provided by a Transmission electron microscope is high,there are certain limitations that makes it not suitable for viewing the motility of a live Treponema.Those limiting factor's are:

  • Instead of a glass lens,an electron beam is used, which is penetrated from above the specimen by using a magnetic lens and projected onto a detector.Since this electrons have a limited penetrating ability, it is not possible to obtain a 3-D view of the specimen.
  • Another important condition for viewing a specimen is that both the beam and speciment should be in a vacuum and the specimen should be very thin and dehydrated.The treatment procedure for making the specimen dehydrated do not allow for viewing a live microbe.Hence, it is not possible to view the motility of a live Treponema.

Dark-Field microscopy is the ideal method used for visualizing a live Treponema,which is otherwise not possible through conventional light microscopy.It can create high-contrast and high-resolution images of specimens, without using any stains and thus eliminating the chances of live specimens getting killed or compromised by the stains.

Thus, we can conclude that , This is the wrong choice because all cells viewed in the TEM will be dead.

Hence,the answer.

gladiator answered 1 year ago
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