Question

You are measuring the titer (i.e. plaque-forming units (PFUs) of the Rabid Wolverine Virus within the bloodstream of Ohio State fans, and you measure the following numbers from blood drawn from one Buckeye (Ohio State) fan. In each case below, you have plated 1 mL of the blood or diluted blood on a plate containing a confluent monolayer of tissue culture cells that are susceptible to the virus. You allowed the plates to incubate overnight, and upon arriving at the lab early the next morning you count the plaques on each plate. Your results are the following:

- the plate with the undiluted blood sample resulted in too many plaques to count

- the plate with the 10-fold dilution of the blood sample resulted in too many plaques to count

- the plate with the 100-fold dilution of the blood sample resulted in approximately 3000 plaques

- the plate with the 1000-fold dilution of the blood sample resulted in 298 plaques counted

- the plate with the 10,000-fold dilution of the blood sample resulted in 32 plaques counted

Which of the following best describes the titer of the Rabid Wolverine Virus within the bloodstream of the single Buckeye (Ohio State) fan you tested?
3 x 10⁹ plaques / mL
3 x 10⁷ plaques / mL
3 x 10¹ plaques / mL
3 x 10⁵ plaques / mL
3 x 10³ plaques / mL

Answer 1