Question

What is the purpose of adding precision plus standard in SDS-PAGE? What will happen if we do not use it in our analysis?

Answer 1

SDS-PAGE is an electrophoresis method that allows protein separation by mass. The medium is a polyacrylamide-based discontinuous gel. In addition, SDS (sodium dodecyl sulfate) is used. About 1.4 grams of SDS bind to a gram of protein, corresponding to one SDS molecule per two amino acids.
Precision Plus Protein Standards are the latest generation of recombinant proteins designed to provide a ladder of convenient consistent sizes. Standards are an integral part of every electrophoresis experiment because they help identify and characterize the molecules separated in a gel. Prestained and unstained MW standards are available for SDS-PAGE, IEF, 2-D PAGE, and western blotting. For migration charts with different types of gels.
Precision Plus Protein™ standards for protein electrophoresis offer lot-to-lot consistency, a good balance between band sharpness and band brightness, accurate MW estimation, and excellent blotting results. These standards contain highly purified recombinant proteins.
If we do not use it on our analysis following features will not be present:
Clean, sharp bands for accurate MW estimation
MW confirmed by mass spectrometry
provides batch-to-batch MW consistency and reproducible electrophoretic migration
Unstained and western standards with a Strep-tag affinity sequence for detection and MW determination on western blots
Natural fluorescence properties for multiplex fluorescent detection