This is the substrate concentration and the kinetic slopes from lab experiment. I'm suppose to construct...

This is the substrate concentration and the kinetic slopes from lab experiment. I'm suppose to construct a graph to estimate Km and Vmax (that's where I need help. How do I do that? Also, I'm suppose to construct another graph of "linear transfored" plot (lineweaver-burk or Eadie-hofstee plots) to determine Km and Vmax. HELP!

[S_o]	v_{o uninhibited}	v_{o inhibited}
0.0833	0.2791	0.0924
0.1667	0.3822	0.1485
0.2500	0.3893	0.2182
0.4167	0.4320	0.3149
0.6667	0.5001	0.4480
0.9167	0.4813	0.5515

Solutions

Expert Solution

by enzyme kinetics equation is

ploting graph between 1/s0 vs 1/v0

intercept gives 1/Vmax and slope gives KM/Vmax

by ploting graphs in excel slope and intercept obtained are

1. uninhibited 1/Vmax = 1.9041 , Vmax =0.525

slope = Km/Vmax = 0.1384, Km = 0.07266

2. inhibited 1/ Vmax = 0.8289, Vmax =1.2064

slope = Km/ Vmax = 1.1643, Km = 1.4046

queen_honey_blossom answered 1 year ago

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