Question

In: Biology

The diagram below represents the DNA loci of the lac operon in E. coli.  Explain the function...

The diagram below represents the DNA loci of the lac operon in E. coli.  Explain the function of each of the loci in the space provided.  Include if the locus is a cis element or a coding region. If a coding region, include what the coding region produces and the protein’s function.  If the locus binds a trans-acting factor, include this as well and the function of the binding.

Y

CAP locus

Lac P locus

Lac Z locus

Lac Y locus

Lac A locus

Lac O locus

Lac I locus

Solutions

Expert Solution

Answer =

Explain the function of each of the loci in the space provided.  Include if the locus is a cis element or a coding region. If a coding region, include what the coding region produces and the protein’s function.  If the locus binds a trans-acting factor, include this as well and the function of the binding.

CAP locus = represents binding site for CAP (catabolite activated protein).

When CAP binds to CAP binding site, it promotes the transcription by helping RNA polymerase bind to the promoter.

is cis acting element.

Lac P locus = reprents the promoter.promoter is the binding site.promoter is the binding site of RNA polymerase, when RNA polymerase binds to the promoter then transcription initiated.

is cis acting element

Lac Z locus - it represents the z gene it codes for beta-galactosidase enzyme which catalyzes the hydrolysis of lactose into glucose and galactose.

it is coding region.

Lac Y locus = it represents galactoside permease.it transports lactose into the cell across the cell membrane.

it is coding region.

Lac A locus - it represents a gene.a gene codes for transacetylase enzyme which helps betagalactosidase enzyme.

it is coding region.

Lac O locus - it represents the operator. operator is the binding site for lac repressor.

cis acting element

it acts as negatively regulating site.when lac repressor bound to operator, RNA polymerase can not bind to the promoter and start transcription.

Lac I locus = represents repressor.

is a trans acting regulator.

it binds to the operator, prevents the binding of RNA polymerase to the promoter.

gladiator answered 1 year ago
Next > < Previous

Related Solutions

Briefly describe the Escherichia coli (E. coli) lac operon and its regulation.
Briefly describe the Escherichia coli (E. coli) lac operon and its regulation.
In E coli the lac operon is used to control the expression of the genes involved...
In E coli the lac operon is used to control the expression of the genes involved in the used and metabolism of lactose . describe how the genes are not expressed in the absence of lactose. describe how the genes are expressed when lactose is present both in the presence and of glucose. what would happen if there were an l-  mutation? what would happen if there were an Oc mutation? which one of those two mutations is recessive ?
What are the specific players in the lac operon in E. coli and S. cerevisiae (trans-acting...
What are the specific players in the lac operon in E. coli and S. cerevisiae (trans-acting factors and cis-elements)?
The lac operon of E. coli encodes the two enzymes ß-Galactosidase and Permease. Indicate in the...
The lac operon of E. coli encodes the two enzymes ß-Galactosidase and Permease. Indicate in the following table whether these enzymes are synthesized (“+”) or not (“–“) given the genotypes in the left column and the presence or absence of the inducer. Note that some of the genotypes are partial diploids, i.e. a second copy of the operon is present on a separate plasmid. Genotype Inducer Absent Inducer Present β-Galactosidase Permease β-Galactosidase Permease a) I+P+O+Z+Y+ b) I+P+O+Z–Y+ c) I+P+O+Z+Y– d)...
For each of the merodiploid E. coli strains containing the lac operon alleles listed, indicate whether...
For each of the merodiploid E. coli strains containing the lac operon alleles listed, indicate whether the strain is inducible, constitutive, or unable to expressβ-galactosidase and permease. Genotype 1) I+ p+ o+ Z- Y+ /I+ oc Z+ Y+ 2) I+ p+ o+ Z+ Y+ /I- p+ oc Z+ Y- 3) I- o+ Z- Y+ /I- p+ oc Z+ Y- 4) I+ p- o+ Z+ Y- /I- p+ oc Z- Y+ this is the problem with no further information
Consider the lac operon in E. coli. a. List the cis-acting elements and the trans-acting factors...
Consider the lac operon in E. coli. a. List the cis-acting elements and the trans-acting factors involved in the regulation of its expression. b. Indicate which Transacting factors interact with which cis-elements. c. Describe what the factors do when they are bound to their respective cis–elements. d. Indicate the inducer in this system and how it works.
he lac operon in E. coli regulates the co-expression of three genes needed for the degradation...
he lac operon in E. coli regulates the co-expression of three genes needed for the degradation of lactose (but absence of glucose) in the growth medium. These genes are not expressed when the bacteria has no lactose to feed on. How does the cell prevent expressing the lac operon in the absence of lactose? How does the presence of lactose activate the transcription of these three genes? (You do not need to explain the glucose level regulation.)
Most of what we know about the lac operon of E. coli has come from genetic...
Most of what we know about the lac operon of E. coli has come from genetic analysis of various mutants. See the following genotype... How much expression would you expect to see in this strain if the growth media has glucose but no lactose present? I+P+O+Z+ CAP- We'll use the numbers associated with transcription amounts as described in lecture and in the problem sets. H ere is the key for these genotypes: A “plus” superscript indicates normal functioning part of...
Most of what we know about the lac operon of E. coli has come from genetic...
Most of what we know about the lac operon of E. coli has come from genetic analysis of various mutants. See the following genotype... How much expression would you expect to see in this strain if the growth media has glucose but no lactose present? I+P+O+Z+ CAP- We'll use the numbers associated with transcription amounts as described in lecture and in the problem sets. H ere is the key for these genotypes: A “plus” superscript indicates normal functioning part of...
or each of the E. coli strains containing the lac operon alleles listed, indicate whether beta-galactosidase...
or each of the E. coli strains containing the lac operon alleles listed, indicate whether beta-galactosidase and permease will be synthesized or not (type yes or no) Genotype Beta-galactosidase Permease Lactose present Lactose absent Lactose present Lactose absent I+ P+ O+ Z- Y+ / I+ P+ Oc Z+ Y+ I+ P+ O+ Z+ Y+ / I- P+ O+ Z+ Y- I- P- O+ Z+ Y- / I+ P+ Oc Z- Y+ Is P+ O+ Z+ Y+ / I- P+ O+...
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT