Which technique would you use to analyze and compare gene expression between normal cells and cancer...

Which technique would you use to analyze and compare gene expression between normal cells and cancer cells to identify differences in mRNA expression between these cell types? Explain each steps reasoning.

Expert Solution

gladiator answered 2 years ago

How can you tell if the gene was highly expressed in cancer cells compared to normal...

How can you tell if the gene was highly expressed in cancer cells compared to normal cells? What about the other way around?

Explain the regulation of gene expression in eukaryotic cells. Discuss mechanisms by which gene expression may...

Explain the regulation of gene expression in eukaryotic cells. Discuss mechanisms by which gene expression may be altered. How do these alterations induce cancer-causing mutations in cell DNA? Explain how cancer is formed. Describe genetic changes found in cancer cells and how these changes lead to alterations in cell behavior. Determine whether proteome data can be utilized in genetic disorder diagnosis. Relate the Human Genome Project data to the analysis of cancer genes.

You design a microarray experiment to determine the difference in gene expression patterns between normal and...

You design a microarray experiment to determine the difference in gene expression patterns between normal and cancerous lung tissue from humans. You label the cDNA derived from a lung tumor with a red fluorescent dye and the cDNA derived from normal lung tissue with a green fluorescent dye. You hybridize the cDNA to a microarray chip in which each spot corresponds to a DNA sequence from a different human gene. a. What can you infer about a particular gene’s expression...

Cell Cancer: a. In what ways are cancer cells different from normal cells in terms of...

Cell Cancer: a. In what ways are cancer cells different from normal cells in terms of properties and behavior? b. What is the difference between an oncogene and a tumor suppressor gene?

Which technique would you use to determine the following? You can only use each technique once....

Which technique would you use to determine the following? You can only use each technique once. Not all techniques will be used. Explain your answer. techniques available: - SDS-PAGE and Western blot - TLC - freeze-fracture - lactoperoxidase labeling - FRAP/photobleaching - photoactivation - hydropathy plot 1. You want to determine if a membrane has proteins in it or not. 2. You want to determine if a cell membrane has the Na+/K+ transporter. 3. You want to determine the types...

Compare and contrast regulation of gene expression with use of an enzyme inhibitor. List one advantage...

Compare and contrast regulation of gene expression with use of an enzyme inhibitor. List one advantage and disadvantage for regulating gene expression and one advantage and one disadvantage for using inhibitor.

Which of the following systems would you use for the expression of an antibody? Explain you...

Which of the following systems would you use for the expression of an antibody? Explain you decision, why you would or would not use: a. CHO b. PERC.6 (human cell line) c. Saccharomyces cerevisiae (yeast) d. E.coli e. Transgenic plant

Which of the following is a difference between prokaryotic and eukaryotic gene expression Group of answer...

Which of the following is a difference between prokaryotic and eukaryotic gene expression Group of answer choices 1)Eukaryotes have basal transcription factors not part of the holoenzyme 2)Eukaryotes produce an immature mRNA 3)Trancription and translation can occur simultaneously in Prok. 4)Eukaryotics have more diverse promoters 5)All of the above

What is the advantage of using double negative 3 cells (DN3) cells to analyse gene expression...

What is the advantage of using double negative 3 cells (DN3) cells to analyse gene expression using real time PCR in leukemia research?

QUESTION 21 Describe the function of FOS in normal cells and cancer cells. For a complete...

QUESTION 21 Describe the function of FOS in normal cells and cancer cells. For a complete answer, you will need to include: A) its mechanism of action, including other proteins it works with and its downstream targets B) how its activity is regulated C) whether it acts as an oncogene or a tumor suppressor and why inactivation or activation of the gene leads to cancer QUESTION 22 Signal Transduction: A) Explain how growth factors, acting through the signal transduction pathway,...

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