Question

Complete the following Table 2.1 for Lab 2.   Reference all the information that you include. The Performance Standards for Antimicrobial Susceptibility Testing 27^th ed. CLSI supplement M100 (by Clinical and Laboratory Standards Institute) is an excellent resource available online and also your Textbook.

Table 2.1 The class, spectrum and mode of action of various antibiotics.

Antibiotic	Antibiotic class	Spectrum of activity (gram +, -, or broad)	Mode (mechanism) of Action	Reference used
Bacitracin	Cyclic polypeptide	Gram +	Inhibition of cell wall formation (peptidoglycan synthesis)	Madigan et. al., 2018, p. 856 and/or Drugbank retrieved June 11, 2019 https://www.drugbank.ca/drugs/DB00626
Colistin
Erythromycin
Nalidixic acid
Vancomycin
Tetracycline
Sulfisoxazole (aka Sulphafurnazole)
Trimethoprim

Answer 1

Antibiotic	Antibiotic class	Spectrum of activity (gram +, -, or broad)	Mode (mechanism) of Action	Reference used
Bacitracin	Cyclic polypeptide	Gram +	Inhibition of cell wall formation (peptidoglycan synthesis)	Madigan et. al., 2018, p. 856 and/or Drugbank retrieved June 11, 2019 https://www.drugbank.ca/drugs/DB00626
Colistin	polymyxin antibiotic	Gram -	endotoxin effect, inhibition of vital respiratory enzymes  in the bacterial inner membrane	https://cmr.asm.org/content/30/2/557/article-info
Erythromycin	macrolide antibiotic	broad spectrum	inhibit protein synthesis by binding to the 23S rRNA molecule (in the 50S subunit) of the bacterial ribosome	http://chemistry.elmhurst.edu/vchembook/654antibiotic.html
Nalidixic acid	quinolone antibacterial agent	Gram -	inhibit bacterial DNA gyrase (topoisomerase II and IV)	Clinical Pharmacology (Eleventh Edition) 2012, Pages 173-190
Vancomycin	glycopeptide antibiotic	broad spectrum	Inhibits cell wall synthesis by binding to D-alanyl-D-alanine residues of the bacterial cell wall.	http://www.antimicrobe.org/drugpopup/Vancomycin.htm
Tetracycline	naphthacene antibiotic	Broad spectrum	inhibition of protein synthesis	https://amrls.cvm.msu.edu/pharmacology/antimicrobials/antibiotics-of-veterinary-importance/tetracyclines
Sulfisoxazole (aka Sulphafurnazole)	sulphonamide	broad-spectrum	inhibits bacterial synthesis of dihydrofolic acid by preventing the condensation of the pteridine with para-aminobenzoic acid (PABA)	https://www.drugbank.ca/drugs/DB14033
trimethoprim is a broad spectrum sulphonamide inhibits bacterial dihydrofolate reductase