a) Why do large proteins come out of a gel filtration column faster than small ones?...

a) Why do large proteins come out of a gel filtration column faster than small ones? Wouldn�t it make more sense for small molecules to move quickly through a gel? Explain.

Expert Solution

Gel filtration chromatography is a separation based on size. It is also called molecular exclusion or gel permeation chromatography. In gel filtration chromatography, the stationary phase consists of porous beads with a well-defined range of pore sizes. The stationary phase for gel filtration is said to have a fractionation range, meaning that molecules within that molecular weight range can be separated.

Gel filtration resin can be thought of as beads which contain pores of a defined size range.
Large proteins which cannot enter these pores pass around the outside of the beads.
Smaller proteins which can enter the pores of the beads have a longer, tortuous path before they exit the bead.
Thus, a sample of proteins passing through a gel filtration column will separate based on molecular size: the big ones will elute first and the smallest ones will elute last (and "middle" sized proteins will elute in the middle).

queen_honey_blossom answered 1 hour ago

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