Question

from chromosome inversion , please describe how to use FISH or CGH technique to determine a disease

Answer 1

Ans) The way to identify process like deletion , translocation , insertion and duplication are probe-based method. Probe-based method is commonly used in FISH ( Fluoresence Insitu Hybridisation). Probe which is integral to sequence of gene interest in the genome are labelled fluoresencent. The parts of chromosomes were stick to the place where sequence is identified. An additional fluoresencent spot is identified during the process of duplication sequence. By nick translation FISH probe can be easily prepared s it needs both DNase and DNA polymerase 1. The function of DNase 1 is established random nicks in the DNA template and DNA polymerase 1 helps to elongate the 3' OH end of nick along the residues of nucleotide and hence , residues are eliminated by the 5' phosphate end. Preparing FISH probe is useful instead of usefullness of commercial aviability of probes. These probes have flexibility for generating probes for any interest sequence as well as about pricing.

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